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Western blot - MAGEA2 antibody - Aminoterminal end (ab71786)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGEA2 antibody - Aminoterminal end (ab71786)
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Product Name
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MAGEA2 antibody - Aminoterminal end
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See all MAGEA2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAGEA2 - Aminoterminal end
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Immunogen
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A KLH conjugated synthetic peptide selected from the N terminal region of human MAGEA2.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 35 kDa plus many other bands (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human breast carcinoma tissue and HL60 lysate.
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Research areas
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Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
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Relevance
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MAGEA2 is an antigen recognized on melanoma by autologous cytolytic T lymphocytes. The function of MAGEA2 is not known, though it may play a role in embryonal development and tumor transformation or aspects of tumor progression. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAGEA2 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAGEA2 antibody - Aminoterminal end images, references, products related to ab71786 and other tools.
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MAGEA2 antibody - Aminoterminal end images:
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 Western blot - MAGEA2 antibody - Aminoterminal end (ab71786)
MAGEA2 antibody - Aminoterminal end (ab71786) at 1/100 dilution + HL60 lysate at 12.5 µg
Predicted band size : 35 kDa
Observed band size : 35 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGEA2 antibody - Aminoterminal end (ab71786)
Formalin fixed, paraffin embedded human breast carcinoma tissue reacted with ab71786 at a 1/50 dilution. ab71786 was peroxidase conjugated to the secondary antibody, followed by AEC staining.
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Search PubMed (MEDLINE) for references to MAGEA2
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MAGEA2 antibody - Aminoterminal end - more information
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