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Products:Cancer >> Tumor immunology >> Tumor-associated antigens
Overview
Product name
Anti-MAGEA2 antibody - Aminoterminal end
See all MAGEA2 products (2) ...
Description
Rabbit polyclonal to MAGEA2 - Aminoterminal end
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide selected from the N terminal region of human MAGEA2.
Positive control
Human breast carcinoma tissue and HL60 lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 35 kDa plus many other bands (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
MAGEA2 is an antigen recognized on melanoma by autologous cytolytic T lymphocytes. The function of MAGEA2 is not known, though it may play a role in embryonal development and tumor transformation or aspects of tumor progression. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
Alternative names
- Cancer/testis antigen 1.2 antibody
- MAGE 2 antibody
- MAGEA2B antibody
see all
Database links
- Entrez Gene: 4101 Human
- Omim: 300173 Human
- SwissProt: P43356 Human
- Unigene: 670781 Human
Anti-MAGEA2 antibody - Aminoterminal end images:
Western blot - MAGEA2 antibody - Aminoterminal end (ab71786)
Anti-MAGEA2 antibody - Aminoterminal end (ab71786) at 1/100 dilution + HL60 lysate at 12.5 µg
Predicted band size : 35 kDa
Observed band size : 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGEA2 antibody - Aminoterminal end (ab71786)
Formalin fixed, paraffin embedded human breast carcinoma tissue reacted with ab71786 at a 1/50 dilution. ab71786 was peroxidase conjugated to the secondary antibody, followed by AEC staining.
References for Anti-MAGEA2 antibody - Aminoterminal end (ab71786)
ab71786 has not yet been referenced specifically in any publications.
Publishing research using ab71786? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"