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Western blot - MAGEA3 antibody (ab22142)
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Product Name
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MAGEA3 antibody
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See all MAGEA3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to MAGEA3
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Immunogen
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Fusion protein: MPLEQRSQHC KPEEGLEARG EALGLVGAQA PATEEQEAAS SSSTLVEVTL GEVPAAESPD PPQSPQGASS LPTTMNYPLW SQSYEDSSNQ EEEGPSTFPD corresponding to amino acids 1/100 of Human MAGEA3
BLAST 'MPLEQRSQHC KPEEGLEARG EALGLVGAQA PATEEQEAAS SSSTLVEVTL GEVPAAESPD PPQSPQGASS LPTTMNYPLW SQSYEDSSNQ EEEGPSTFPD ' with  or 
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 35 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
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Research areas
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Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The function of MAGEA3 is unknown, although it may play a role in embryonal development and tumor transformation or aspects of tumor progression.
This antigen is recognized on a melanoma by autologous cytolytic T lymphocytes. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. It is never expressed in kidney tumors, Leukemias and lymphomas.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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MAGEA3 antibody (ab22142) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Constituents: 50% Glycerol Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Notes
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Produced from outbred CD1 mice
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAGEA3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAGEA3 antibody images, references, products related to ab22142 and other tools.
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MAGEA3 antibody images:
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 Western blot - MAGEA3 antibody (ab22142)
All lanes : MAGEA3 antibody (ab22142) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 35 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
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Search PubMed (MEDLINE) for references to MAGEA3
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MAGEA3 antibody - more information
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Customer reviews (feedback) regarding MAGEA3 antibody |
Customer FAQs regarding MAGEA3 antibody |
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Price and availability of products related to MAGEA3 antibody |
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MAGEA3 antibody - related products:
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MAGEA3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAGEA3 antibody |
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