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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-MAGEA3 antibody
See all MAGEA3 products (4) ...
Description
Rabbit polyclonal to MAGEA3
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (10-30 amino acids in length) conjugated to KLH, from the C terminal of the last 50 amino acids of Human MAGEA3.
Positive control
Jurkat lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Western blot - MAGEA3 antibody (ab38496)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab38496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Target
Relevance
MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The function of MAGEA3 is unknown, although it may play a role in embryonal development and tumor transformation or aspects of tumor progression. This antigen is recognized on a melanoma by autologous cytolytic T lymphocytes. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. It is never expressed in kidney tumors, Leukemias and lymphomas.
Alternative names
- CT1.3 antibody
- MGC1461 antibody
- Antigen MZ2 D antibody
see all
Database links
- Entrez Gene: 4102 Human
- Omim: 300174 Human
- SwissProt: P43357 Human
- Unigene: 417816 Human
Anti-MAGEA3 antibody images:
Western blot - MAGEA3 antibody (ab38496)
Anti-MAGEA3 antibody (ab38496) at 1/100 dilution + Jurkat lysate
Predicted band size : 35 kDa
Observed band size : 35 kDa
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
References for Anti-MAGEA3 antibody (ab38496)
ab38496 has not yet been referenced specifically in any publications.
Publishing research using ab38496? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"