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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-MAGEA3 antibody
See all MAGEA3 products (4) ...
Description
Mouse polyclonal to MAGEA3
Specificity
This antibody reacts with MAGEA3.
Tested applications
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Recombinant fusion protein: WSQSYEDSSNQEEEGPSTFP, corresponding to amino acids 80-99 of Human MAGEA3
WSQSYEDSSN QEEEGPSTFP
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Western blot - MAGEA3 antibody (ab52706)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Detects a band of approximately 32.2 kDa (predicted molecular weight: 35 kDa).
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The function of MAGEA3 is unknown, although it may play a role in embryonal development and tumor transformation or aspects of tumor progression. This antigen is recognized on a melanoma by autologous cytolytic T lymphocytes. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. It is never expressed in kidney tumors, Leukemias and lymphomas.
Alternative names
- CT1.3 antibody
- MGC1461 antibody
- Antigen MZ2 D antibody
see all
Database links
- Entrez Gene: 4102 Human
- Omim: 300174 Human
- SwissProt: P43357 Human
- Unigene: 417816 Human
Anti-MAGEA3 antibody images:
Western blot - MAGEA3 antibody (ab52706)
All lanes : Anti-MAGEA3 antibody (ab52706) at 1/1000 dilution
Lane 1 : Total protein extract from E coli with ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen.
Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag).
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 35 kDa
Observed band size : 32.2 kDa (why is the actual band size different from the predicted?)
Note: the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.
References for Anti-MAGEA3 antibody (ab52706)
ab52706 has not yet been referenced specifically in any publications.
Publishing research using ab52706? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"