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Western blot - MAGEA9 antibody (ab38493)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGEA9 antibody (ab38493)
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Product Name
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MAGEA9 antibody
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See all MAGEA9 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAGEA9
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Immunogen
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A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 211~223 of human MAGEA9.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific for MAGEA9.
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
IHC-P: Use at an assay dependent dilution.
WB: 1/100 - 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Breast carcinoma tissue and Placental lysate.
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Research areas
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Cancer >> Tumor immunology >> Tumor-associated antigens
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Relevance
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MAGEA9 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.
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MAGEA9 antibody (ab38493) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAGEA9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAGEA9 antibody images, references, products related to ab38493 and other tools.
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MAGEA9 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGEA9 antibody (ab38493)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38493 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
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Search PubMed (MEDLINE) for references to MAGEA9
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MAGEA9 antibody - more information
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Customer reviews (feedback) regarding MAGEA9 antibody |
Customer FAQs regarding MAGEA9 antibody |
Protocols for MAGEA9 antibody |
Price and availability of products related to MAGEA9 antibody |
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MAGEA9 antibody - related products:
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MAGEA9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAGEA9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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