Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cancer >> Tumor immunology >> Tumor-associated antigens
Overview
Product name
Anti-MAGEA9 antibody
See all MAGEA9 products (3) ...
Description
Rabbit polyclonal to MAGEA9
Specificity
This antibody is specific for MAGEA9.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 211~223 of human MAGEA9.
Positive control
Breast carcinoma tissue and Placental lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
IHC-P: Use at an assay dependent dilution.
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Tissue specificity
Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
Sequence similarities
Contains 1 MAGE domain.
Target information above from: UniProt accessionP43362
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cancer/testis antigen 1.9 antibody
- CT1.9 antibody
- MAGA9_HUMAN antibody
see all
Database links
- Entrez Gene: 4108 Human
- Entrez Gene: 728269 Human
- Omim: 300342 Human
- SwissProt: P43362 Human
- Unigene: 460974 Human
- Unigene: 512582 Human
Anti-MAGEA9 antibody images:
Western blot - MAGEA9 antibody (ab38493)
Anti-MAGEA9 antibody (ab38493) at 1/100 dilution + Placenta lysate
Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGEA9 antibody (ab38493)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38493 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
References for Anti-MAGEA9 antibody (ab38493)
ab38493 has not yet been referenced specifically in any publications.
Publishing research using ab38493? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"