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Western blot - MAGEA9 antibody (ab49074)
Western blot - MAGEA9 antibody (ab49074)
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Product Name 

MAGEA9 antibody

 

See all MAGEA9 antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MAGEA9

Immunogen 

A region within synthetic peptide: SLEQRSPHCK PDEDLEAQGE DLGLMGAQEP TGEEEETTSS SDSKEEEVSA, corresponding to N terminal amino acids 2-51 of Human MAGEA9
BLAST 'SLEQRSPHCK PDEDLEAQGE DLGLMGAQEP TGEEEETTSS SDSKEEEVSA ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre: 1/62500.
WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HepG2 cell lysate

Research areas 

Cancer >> Tumor immunology >> Tumor-associated antigens

Relevance 

MAGEA9 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.

 

MAGEA9 antibody (ab49074)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

4108    (Human)

Omim

    

300342    (Human)

SwissProt

    

P43362    (Human)

Unigene

    

512582    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAGEA9 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for MAGEA9 antibody images, references, products related to ab49074 and other tools.

 

MAGEA9 antibody images:

Product image  Western blot - MAGEA9 antibody (ab49074)

Western blot - MAGEA9 antibody (ab49074)

Lane 1 : molecular weight markers
Lane 2 : MAGEA9 antibody (ab49074) at 2.5 µg/ml

Lane 1 : molecular weight markers
Lane 2 : HepG2 cell lysate at 10 µg

Secondary
Lane 1 : molecular weight markers
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50,000 to 1/100,000 dilution


Predicted band size : 35 kDa
Observed band size : 35 kDa

 
 

Search PubMed (MEDLINE) for references to MAGEA9

 

MAGEA9 antibody - more information

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