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Products:Cancer >> Tumor immunology >> Tumor-associated antigens
Overview
Product name
Anti-MAGEA9 antibody
See all MAGEA9 products (3) ...
Description
Rabbit polyclonal to MAGEA9
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
A region within synthetic peptide: SLEQRSPHCK PDEDLEAQGE DLGLMGAQEP TGEEEETTSS SDSKEEEVSA, corresponding to N terminal amino acids 2-51 of Human MAGEA9
SLEQRSPHCK PDEDLEAQGE DLGLMGAQEP TGEEEETTSS SDSKEEEVSA
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Western blot - MAGEA9 antibody (ab49074)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre: 1/62500.
WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Tissue specificity
Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
Sequence similarities
Contains 1 MAGE domain.
Target information above from: UniProt accessionP43362
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cancer/testis antigen 1.9 antibody
- CT1.9 antibody
- MAGA9_HUMAN antibody
see all
Database links
- Entrez Gene: 4108 Human
- Entrez Gene: 728269 Human
- Omim: 300342 Human
- SwissProt: P43362 Human
- Unigene: 460974 Human
- Unigene: 512582 Human
Anti-MAGEA9 antibody images:
Western blot - MAGEA9 antibody (ab49074)
Lane 1 : molecular weight markers
Lane 2 : Anti-MAGEA9 antibody (ab49074) at 2.5 µg/ml
Lane 1 : molecular weight markers
Lane 2 : HepG2 cell lysate at 10 µg
Secondary
Lane 1 : molecular weight markers
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50,000 to 1/100,000 dilution
Predicted band size : 35 kDa
Observed band size : 35 kDa
References for Anti-MAGEA9 antibody (ab49074)
ab49074 has not yet been referenced specifically in any publications.
Publishing research using ab49074? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"