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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-MAGED1 antibody
See all MAGED1 products (4) ...
Description
Rabbit polyclonal to MAGED1
Specificity
This antibody is specific for MAGED1.
Tested applications
IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 466~481 of human MAGED1.
Positive control
A549 lysate and breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500.Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds p75NTR and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate p53 transcriptional activity and inhibit cell proliferation. Enhances p53 phosphorylation and accumulation.
Tissue specificity
Expressed in bone marrow stromal cells from both multiple myeloma patients and healthy donors. Seems to be ubiquitously expressed.
Sequence similarities
Contains 1 MAGE domain.
Cellular localization
Cytoplasm. Cell membrane. Expression shifts from the cytoplasm to the plasma membrane upon stimulation with NGF.
Target information above from: UniProt accessionQ9Y5V3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DLXIN 1 antibody
- MAGD1_HUMAN antibody
- MAGE D1 antigen antibody
see all
Database links
- Entrez Gene: 9500 Human
- Omim: 300224 Human
- SwissProt: Q9Y5V3 Human
- Unigene: 5258 Human
Anti-MAGED1 antibody images:
Western blot - MAGED1 antibody (ab38492)
Anti-MAGED1 antibody (ab38492) at 1/100 dilution + A549 lysate
Predicted band size : 86 kDa
Observed band size : 86 kDa
Additional bands at : 30 kDa,45 kDa,50 kDa,55 kDa,60 kDa,65 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGED1 antibody (ab38492)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38492, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.
References for Anti-MAGED1 antibody (ab38492)
ab38492 has not yet been referenced specifically in any publications.
Publishing research using ab38492? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"