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Anti-MAGED1 antibody (ab77045)

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Overview

Product name

Anti-MAGED1 antibody
See all MAGED1 products (4) ...

Description

Mouse monoclonal to MAGED1

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment: EMADIQVSAA AARPKSAFKV QNATTKGPNG VYDFSQAHNA KDVPNTQPKA AFKSQNATPK GPNAAYDFSQ AATTGELAAN KSEMAFKAQN ATTKVGPNAT YNFSQSLNAN , corresponding to amino acids 117-227 of Human MAGED1 (NP_001005333) with a 26kDa enzyme tag

EMADIQVSAA AARPKSAFKV QNATTKGPNG VYDFSQAHNA KDVPNTQPKA AFKSQNATPK GPNAAYDFSQ AATTGELAAN KSEMAFKAQN ATTKVGPNAT YNFSQSLNAN

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Jurkat cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 1X PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Isotype

IgG2b

Light chain type

kappa

Research areas

Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

  • Western blot - MAGED1 antibody [Not required] (ab77045)Western blot - MAGED1 antibody [Not required] (ab77045) image (enlarge)

  • Western blot - MAGED1 antibody [Not required] (ab77045)Western blot - MAGED1 antibody [Not required] (ab77045) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab77045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide2 Images

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

Target

Function

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds p75NTR and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate p53 transcriptional activity and inhibit cell proliferation. Enhances p53 phosphorylation and accumulation.

Tissue specificity

Expressed in bone marrow stromal cells from both multiple myeloma patients and healthy donors. Seems to be ubiquitously expressed.

Sequence similarities

Contains 1 MAGE domain.

Cellular localization

Cytoplasm. Cell membrane. Expression shifts from the cytoplasm to the plasma membrane upon stimulation with NGF.

Target information above from: UniProt accessionQ9Y5V3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DLXIN 1 antibody
  • MAGD1_HUMAN antibody
  • MAGE D1 antigen antibody
  • MAGE tumor antigen CCF antibody
  • MAGE-D1 antigen antibody
  • Maged1 antibody
  • Melanoma antigen family D 1 antibody
  • Melanoma associated antigen D1 antibody
  • Melanoma-associated antigen D1 antibody
  • Neurotrophin receptor interacting MAGE homolog antibody
  • Neurotrophin receptor-interacting MAGE homolog antibody
  • NRAGE antibody
  • PP2250 antibody
see all

Database links

Anti-MAGED1 antibody images:

  Western blot - MAGED1 antibody [Not required] (ab77045)

Western blot - MAGED1 antibody [Not required] (ab77045)

Anti-MAGED1 antibody (ab77045) at 1/500 dilution + Jurkat cell lysate at 25 µg

Secondary
HRP-conjugated goat anti-mouse IgG at 1/2500 dilution
developed using the ECL technique

Predicted band size : 86 kDa
Observed band size : 86 kDa

  Western blot - MAGED1 antibody [Not required] (ab77045)

Western blot - MAGED1 antibody [Not required] (ab77045)

Anti-MAGED1 antibody (ab77045) at 1/500 dilution + immunogen (38.21 kDa) at 0.1 µg

Secondary
HRP-conjugated goat anti-mouse IgG at 1/5000 dilution
developed using the ECL technique

Predicted band size : 86 kDa


Predicted band size of the fragment used as the immunogen: 38.21kDa

References for Anti-MAGED1 antibody (ab77045)

ab77045 has not yet been referenced specifically in any publications.

Publishing research using ab77045? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"