MAGI3 antibody [SLP-32] (ab11509)

Product Name 

MAGI3 antibody [SLP-32]

Product type 

Primary antibodies

Description 

Mouse monoclonal [SLP-32] to MAGI3

Immunogen 

Synthetic peptide, corresponding to C-term amino acids 421-785 of Rat MAGI3.

Reacts with 
(species key)

Rat

Specificity 

MAGI3 (membrane-associated guanylate cyclase of inverted orientation-3) is a member of the family of membrane-associated guanylate kinases (MAGUKs) that comprise peripheral membrane proteins involved in the formation of specialized cell-cell junctions. MAGI3, as well as other proteins in this family are characterized by three types of protein-protein interaction modules: five PDZ domains, an N-terminal guanylate kinase (GK) domain, and a WW domain. The WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, mediates protein-protein interactions through binding to short proline-rich sequences). MAGI3 and PTEN/MMAC together modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions PTEN/MMAC phosphatase to specific subcellular locations that are involved in the regulation of cell proliferation and survival.

Tested applications 
(see key)

ELISA, ICC, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
WB: Use at a concentration of 1-2 µg/ml. Detects a band of approximately 160 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tested with rat brain extract.

Cellular localization 

Cell Membrane

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial

Relevance 

MAGI3 (membrane-associated guanylate kinase of inverted orientation-3) is a member of the family of membrane-associated guanylate kinases (MAGUKs) that comprise peripheral membrane proteins involved in the formation of specialized cell-cell junctions. MAGI3, as well as other proteins in this family are characterized by three types of protein-protein interaction modules: five PDZ domains, an N-terminal guanylate kinase (GK) domain, and a WW domain. The WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, mediates protein-protein interactions through binding to short proline-rich sequences). MAGI3 and PTEN/MMAC together modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions PTEN/MMAC phosphatase to specific subcellular locations that are involved in the regulation of cell proliferation and survival.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

SLP-32

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 1.70 - 1.70 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Customer reviews (feedback) regarding MAGI3 antibody [SLP-32]

Customer FAQs regarding MAGI3 antibody [SLP-32]

Protocols for MAGI3 antibody [SLP-32]

Price and availability of products related to MAGI3 antibody [SLP-32]

MAGI3 antibody [SLP-32] - related products: