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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
Overview
Product name
Anti-MAGI3 antibody [SLP-32]
See all MAGI3 products (3) ...
Description
Mouse monoclonal [SLP-32] to MAGI3
Specificity
MAGI3 (membrane-associated guanylate cyclase of inverted orientation-3) is a member of the family of membrane-associated guanylate kinases (MAGUKs) that comprise peripheral membrane proteins involved in the formation of specialized cell-cell junctions. MAGI3, as well as other proteins in this family are characterized by three types of protein-protein interaction modules: five PDZ domains, an N-terminal guanylate kinase (GK) domain, and a WW domain. The WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, mediates protein-protein interactions through binding to short proline-rich sequences). MAGI3 and PTEN/MMAC together modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions PTEN/MMAC phosphatase to specific subcellular locations that are involved in the regulation of cell proliferation and survival.
Tested applications
ELISA, WB, ICCmore details
Cross reactivity
Reacts with
Rat
Immunogen
Synthetic peptide, corresponding to C-term amino acids 421-785 of Rat MAGI3.
Positive control
Tested with rat brain extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
SLP-32
Isotype
IgG1
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1-2 µg/mlDetects a band of approximately 160 kDa.
ICC: Use at an assay dependent dilution.
Target
Function
Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 6 PDZ (DHR) domains.
Contains 2 WW domains.
Post-translational
modifications
Ubiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex.
Cellular localization
Cell membrane. Cell junction > tight junction. Nucleus. Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.
Target information above from: UniProt accessionQ5TCQ9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- WW and PDZ domain-containing protein 3 antibody
- MAGI-3 antibody
- magi3 antibody
see all
Database links
References for Anti-MAGI3 antibody [SLP-32] (ab11509)
ab11509 has not yet been referenced specifically in any publications.
Publishing research using ab11509? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"