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All lanes : Anti-MAGOH antibody [21B12] (ab10686) at 5 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 17.5 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Anti-MAGOH antibody [21B12] (ab10686) at 1/1000 dilution + whole cell lysate prepared from murine embryonic fibroblasts at 30 µg
Secondary
Goat anti-mouse monoclonal conjugated to IRDye 800CW at 1/15000 dilution
Predicted band size : 17.5 kDa
Image courtesy of an anonymous Abreview.
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