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Western blot - MAGOH antibody [21B12] (ab10686)
![Western blot - MAGOH antibody [21B12] (ab10686)](/ps/datasheet/images/10/ab10686/MAGOH-Primary-antibodies-ab10686-2.jpg)
(enlarge)
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Product Name
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MAGOH antibody [21B12]
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See all MAGOH antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [21B12] to MAGOH
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Immunogen
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Full lenght fusion protein (Human).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 17.5 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Raji cell extract.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
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Relevance
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Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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21B12
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Myeloma
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Sp2/0
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Isotype
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IgG2a
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified antibody (from supernatant).
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAGOH antibody [21B12] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAGOH antibody [21B12] images, references, products related to ab10686 and other tools.
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MAGOH antibody [21B12] images:
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 Western blot - MAGOH antibody [21B12] (ab10686)
All lanes : MAGOH antibody [21B12] (ab10686) at 5 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 17.5 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to MAGOH
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MAGOH antibody [21B12] - more information
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Customer reviews (feedback) regarding MAGOH antibody [21B12] |
Customer FAQs regarding MAGOH antibody [21B12] |
Protocols for MAGOH antibody [21B12] |
Price and availability of products related to MAGOH antibody [21B12] |
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MAGOH antibody [21B12] - related products:
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MAGOH antibody [21B12] - other tools:
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Contact Abcam with a Technical Enquiry about MAGOH antibody [21B12] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - MAGOH antibody [21B12] (ab10686)](/index.html?datasheet=10686#MAGOH-Primary-antibodies-ab10686-2.jpg)
