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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab38768? Please let us know so that we can cite the reference in this datasheet
ab38768 has been referenced in 1 publications.
- Silver DL et al. The exon junction complex component Magoh controls brain size by regulating neural stem cell division. Nat Neurosci 13:551-8 (2010). WB; Mouse. PubMed: 20364144
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - MAGOH antibody (ab38768)
ab38768 staining MAGOH in HeLa cells (green). Cells were fixed in Paraformaldehyde and counterstained with DAPI (red). Please refer to abreview for further details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGOH antibody (ab38768)
IHC image of MAGOH staining in human cerebral cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38768, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - MAGOH antibody (ab38768)
All lanes : Anti-MAGOH antibody (ab38768) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 17 kDa
Observed band size : 17 kDa
Additional bands at : 26 kDa,37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
MAGOH antibody for ICC/IF in Human (38768)
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