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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-MAL antibody
Description
Rabbit polyclonal to MAL
Tested applications
IHC-P, WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Rat, Dog
Immunogen
Synthetic peptide: CFDGFTY(R/K)HYHENI, corresponding to amino acids 114-126 of rat and human MAL.
CFDGFTY(R/ K)HYHENI
Positive control
Rat kidney extracts.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Cell Biology >> Cell Cycle >> Cell differentiation
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MAL antibody(ab15418)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab15418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 10 µg/mlPerform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB: 1/500Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa).
ICC/IF: Use at an assay dependent concentration. (PubMed: 20861303)
IP: Use at an assay dependent concentration. (PubMed: 20861303)
Target
Function
Could be an important component in vesicular trafficking cycling between the Golgi complex and the apical plasma membrane. Could be involved in myelin biogenesis and/or myelin function.
Sequence similarities
Belongs to the MAL family.
Contains 1 MARVEL domain.
Developmental stage
Expressed in the intermediate and late stages of T-cell differentiation.
Post-translational
modifications
Lipoprotein.
Cellular localization
Membrane.
Target information above from: UniProt accessionP21145
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mal antibody
- MAL protein gene antibody
- Mal T-cell differentiation protein antibody
see all
Database links
Anti-MAL antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MAL antibody(ab15418)
IHC image of ab15418 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab15418, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-MAL antibody (ab15418)
This product has been referenced in:
- Carmosino Met al. MAL/VIP17, a new player in the regulation of NKCC2 in the kidney. Mol Biol Cell 21:3985-97 (2010). ICC/IF, IP; Rat, Dog.Read more (PubMed: 20861303) »
- Kamsteeg EJet al. MAL decreases the internalization of the aquaporin-2 water channel. Proc Natl Acad Sci U S A 104:16696-701 (2007).Read more (PubMed: 17940053) »
See all 2 publications for this product
Publishing research using ab15418? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"