Anti-MAP1B antibody [3G5] - Neuronal Marker (ab3095)

I suspect this antibody might be the same as the 3G5 clone for pericytes, deposited as ATCC Number# CRL-1814 and first described by Nayak , which recognizes O-acetylated disialoganglioside present on a wide variety of endocrine and neuronal tissues, and pericytes. I realize your MAP5 antibody is presently offered as an antibody against a microtubule-associated protein, but I wonder if neuronal reactivity to MAP5 was determined after reactivity to pericytes was described, and this antibody henceforth was recognized mainly as such, though the antigens recognized would be very different. It would be a coincidence that this clone number (3G5), is associated with two separate antibodies to neural tissues. Could you please track down the origin of the hybridoma for this antibody to rule out the hypothesis this antibody is the same 3G5 clone deposited by Nayak ? If it is not, would there be any interest in offering Nayak's 3G5 antibody to pericytes?

ANSWER:

We have not found any references for this antibody yet and are still looking. Definitely these two clones with the same designation are different. MAP1B/MAP5 (microtubule associated protein) stains neurons and the epitope is not determined. This is an IgG1 antibody. The acetylated disialogangliside is IgM antibody and is associated with endocrine cells.

If we find out any more information or references I will send those to you, I hope the information above will already help,

I am currently in the process of primary culturing bovine cerebral pericytes. As you may know, pericytes can be difficult to identify by means of a specific marker in culture. I have come across 3G5 in the literature, which is a gangloside antigen that can be used as a pericyte specific marker. Is your antibody MAP5 antibody [3G5]-Neuronal Marker (ab3095) a spcific marker for the 3G5 expressed in pericytes?

ANSWER:

Thank you for your enquiry. For this antibody, 3G5 refers to the clone number. The immunnogen used to generate this antibody is purified bovine brain MAP's (Microtubule Associated Protein) so I don't think this is what you want. By doing a web search, I came across the following pericyte markers which you may want to look at: Desmin; Angiopoietin-1; Osteonectin, among others. I also saw a reference to the 3G5 antigen that you were referring to: 3G5 ganglioside antigen (Nayak, et al., 1988) Pericytes in culture.