Anti-MAP1B antibody [3G5] - Neuronal Marker (ab3095)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAP1B antibody [3G5] - Neuronal Marker (ab3095)

The image illustrating high definition dendritic staining but very limited cell soma staining. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Suzanne Campion (Southampton University).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAP1B antibody [3G5] - Neuronal Marker (ab3095)

ab3095 staining MAP1B in human small intestine tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed. The primary antibody was diluted 1/1600 and incubated with the sample for 20 minutes at 25°C. A HRP conjugated goat monoclonal was used as the secondary.

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence-MAP1B antibody [3G5] - Neuronal Marker(ab3095)

ICC/IF image of ab3095 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3095, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Flow Cytometry-Anti-MAP1B antibody [3G5] - Neuronal Marker(ab3095)

Overlay histogram showing SH-SY5Y cells stained with ab3095 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3095, 1µg/1x10⁶ cells dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.