MAP1B antibody [3G5] - Neuronal Marker (ab3095)

Product Name 

MAP1B antibody [3G5] - Neuronal Marker

Product type 

Primary antibodies

Description 

Mouse monoclonal [3G5] to MAP1B - Neuronal Marker

Immunogen 

Purified bovine brain MAP's.

Reacts with 

Hu, Ms, Rat, Cow

Does not react with 
(species key)

Chk

Tested applications 
(see key)

ICC/IF, IHC (PFA fixed), IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 - 2 µg/ml for 30mins at RT. Do not perform antigen retrieval.
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution (see Pang et al reference). Tissue was perfusion fixed in 4%PFA, then postfixed for 24hrs in the same solution, then cryoprotected in 30% sucrose and cut on a cryostat as free floating sections. Tissue sections were incubated overnight at RT with primary antibody, washed, then incubated with a biotin conjugated antibody, then ABC kit and stainig was performed with DAB as peroxidase substrate.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Brain.

Research areas 

Neuroscience >> Cell Type Marker >> Neuron marker >> Axon marker
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Neuronal
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules

Relevance 

Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5.
MAP1B is the major microtubule associated protein in developing brain which changes its expression during development. In the newborn rat brain, it is a major component of microtubules but in the adult its level is ten fold lower. This change in the level of expression occurs simultaneously with neuronal maturation. MAP1B is the first MAP to appear in growing axons during development as it is present from the first emergence of the nascent axon from the cell body.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

3G5

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

See below for MAP1B antibody [3G5] - Neuronal Marker images, references, products related to ab3095 and other tools.

MAP1B antibody [3G5] - Neuronal Marker images:

References for MAP1B antibody [3G5] - Neuronal Marker (ab3095)

  This clone has been used in: (two most recent references)

Allen E et al. Gigaxonin-controlled degradation of MAP1B light chain is critical to neuronal survival. Nature 438:224-8 (2005). PubMed: 16227972

Gong CX et al. Phosphorylation of microtubule-associated protein tau is regulated by protein phosphatase 2A in mammalian brain. Implications for neurofibrillary degeneration in Alzheimer's disease. J Biol Chem 275:5535-44 (2000). PubMed: 10681533

See all 4 publication references for this product.


If you publish research using ab3095 please let us know so that we can cite the reference on this datasheet.

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