Anti-MAP1B antibody [AA6] (ab11266)

Some time ago I bought your monoclonal MAP1b-AA6 antibody, lot no. 160043. I have previously used the antibody to detect MAP1b on western blots. However, this new shipment of has not given the expected results, the blots come out blank. Do you know if there might be a problem with this specific lot?

ANSWER:

Thank you for contacting us for technical support. I'm sorry to hear you are experiencing problems with your recent vial of ab11266. We have not received any other complaints about this very popular antibody. Could you please provide your order number and I will look into possible shipping delays which would indicate a damage during shipping or storage? The antibody is made as ascites (i.e. unpurified antibody) therefore the concentration of antibody varies from lot to lot and you may need to vary the dilution of the antibody.

If you could please tell me what dilutions you have tried and incubation times, this would help me understand your problem.

I look forward to hearing from you,

Does this antibody recognize the light or heavy chain of MAP1b? It cannot recognize both.

ANSWER:

Thank you for your enquiry. I have received the following information from our source for this antibody, which I hope will be useful. I have added the publications listed below, along with the PubMed links, to the online datasheet for ab11266.

"This antibody, clone AA6, recognizes a conserved nonphosphorylated and nonphosphorylatable epitope on MAP1b. See: Paglini, G., et al., Evidence for the Participation of the Neuron-Specific CDK5 Activator P35 during Laminin-Enhanced Axonal Growth. J. Neuroscience, 18(23), 9858-9869 (1998). DiTella, M., et al., MAP1B/tau functional redundancy during laminin-enhanced axonal growth. J. Cell. Sci., 109, 467-477 (1996).

It reacts with all isoforms of MAP1b, in both Western blot and immunofluorescence applications. See: Franzen, R., et al., Microtubule-associated protein 1B: a neuronal binding partner for myelin-associated glycoprotein. J. Cell Biol., 155(6), 893-898 (2001).

I searched all three of the papers cited above, but I did not see any mention of heavy or light chains of MAP1B. In particular, the Franzen paper shows several Western blots with a band that has a MW of about 250 kDa, which I think is the "full size" MAP1B. I'm curious...what was the indication that MAP1B has a heavy chain and light chain?"

Thank you, and have a great weekend!

What is the specific immunogen for the AA6 antibody? Is this antibody specific for the light chain LC1?

ANSWER:

Thank you for your enquiry.

I have contacted our lab and I have discovered that given that this antibody was raised using rat brain microtubule associated proteins and unfortunately there has been no follow up to map the epitope.

I also performed some searching using the EXPASY database and correct me if I am wrong but it appears that the domains of the light and heavy chains are yet to be defined. (see Key Features at: http://ca.expasy.org/uniprot/P15205).

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

can MAP1b (ab11266) be used to localize pericytes?

thank you

ANSWER:

Thank you for your enquiry and your interest in our products.

This antibody has been tested and characterized using newborn rat brain. Unfortunately, we have not particularly tested in Pericytes.