Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab46801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16, 18 kDa (predicted molecular weight: 14 kDa).Can be blocked with Rat MAP1LC3A peptide (ab64589) or Rat MAP1LC3A peptide (ab64590).

Target

  • FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificityMost abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the MAP1 LC3 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • APG8 antibody
    • ATG8E antibody
    • Autophagy related protein LC3 A antibody
    • Autophagy related ubiquitin like modifier LC3 A antibody
    • Autophagy-related protein LC3 A antibody
    • Autophagy-related ubiquitin-like modifier LC3 A antibody
    • LC3 antibody
    • LC3A antibody
    • MAP1 light chain 3 like protein 1 antibody
    • MAP1 light chain 3-like protein 1 antibody
    • MAP1A/1B light chain 3 A antibody
    • MAP1A/MAP1B LC3 A antibody
    • MAP1A/MAP1B light chain 3 A antibody
    • MAP1ALC3 antibody
    • MAP1BLC3 antibody
    • Map1lc3a antibody
    • Microtubule associated protein 1 light chain 3 alpha antibody
    • Microtubule associated proteins 1A/1B light chain 3 antibody
    • Microtubule associated proteins 1A/1B light chain 3A antibody
    • Microtubule-associated protein 1 light chain 3 alpha antibody
    • Microtubule-associated proteins 1A and 1B, light chain 3 antibody
    • Microtubule-associated proteins 1A/1B light chain 3A antibody
    • MLP3A antibody
    • MLP3A_HUMAN antibody
    see all

Anti-MAP1LC3A antibody images

  • All lanes : Anti-MAP1LC3A antibody (ab46801) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 16,18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa,75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MAP1LC3A antibody (ab46801)

ab46801 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"