Anti-MAP1LC3A antibody (ab46801)

Overview

• Product nameAnti-MAP1LC3A antibodySee all MAP1LC3A primary antibodies ...
• Description
  Rabbit polyclonal to MAP1LC3A
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat MAP1LC3A.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab64589.)

• Positive controlThis antibody gave a positive signal in the following Lysates: Mouse Brain Tissue PC12 Whole Cell HeLa Whole Cell

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Autophagy and mitophagy
  • Autophagosome

  • Cancer
  • Signal transduction
  • Autophagy

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microtubules
  • MT Associated Proteins
  • MAP

  • Cardiovascular
  • Heart
  • Autophagy
  • APG gene products

  • Cardiovascular
  • Heart
  • Autophagy
  • Autophagosome

  • Microbiology
  • Organism
  • Eukaryotic Microorganism
  • Yeast
  • Saccharomyces spp

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ub-like Proteins

Applications

Target

• FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
• Tissue specificityMost abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
• Sequence similaritiesBelongs to the MAP1 LC3 family.
• Post-translational
  modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
• Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.

Target information above from: UniProt accession Q9H492 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 84557 Human
  • Entrez Gene: 66734 Mouse
  • Entrez Gene: 362245 Rat
  • Omim: 601242 Human
  • SwissProt: Q9H492 Human
  • SwissProt: Q91VR7 Mouse
  • SwissProt: Q6XVN8 Rat
  • Unigene: 632273 Human

  • Unigene: 196239 Mouse
  • Unigene: 3135 Rat

  see all

• Alternative names
  APG8 antibodyATG8E antibodyAutophagy related protein LC3 A antibody

  Autophagy related ubiquitin like modifier LC3 A antibodyAutophagy-related protein LC3 A antibodyAutophagy-related ubiquitin-like modifier LC3 A antibodyLC3 antibodyLC3A antibodyMAP1 light chain 3 like protein 1 antibodyMAP1 light chain 3-like protein 1 antibodyMAP1A/1B light chain 3 A antibodyMAP1A/MAP1B LC3 A antibodyMAP1A/MAP1B light chain 3 A antibodyMAP1ALC3 antibodyMAP1BLC3 antibodyMap1lc3a antibodyMicrotubule associated protein 1 light chain 3 alpha antibodyMicrotubule associated proteins 1A/1B light chain 3 antibodyMicrotubule associated proteins 1A/1B light chain 3A antibodyMicrotubule-associated protein 1 light chain 3 alpha antibodyMicrotubule-associated proteins 1A/1B light chain 3A antibodyMLP3A antibodyMLP3A_HUMAN antibody

  see all

Anti-MAP1LC3A antibody images

• 

  Western blot - MAP1LC3A antibody (ab46801)
  All lanes : Anti-MAP1LC3A antibody (ab46801) at 1 µg/ml
  
  Lane 1 : Brain (Mouse) Tissue Lysate
  Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
  Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 14 kDa
  Observed band size : 16,18 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 55 kDa,75 kDa. We are unsure as to the identity of these extra bands.