MAP1LC3A protein (Tagged-His Tag) (ab92050)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab92050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- ATG8EAutophagy-related protein LC3 AAutophagy-related ubiquitin-like modifier LC3 A
- LC3LC3AMAP1 light chain 3 like protein 1MAP1 light chain 3-like protein 1MAP1A/1B light chain 3 AMAP1A/MAP1B LC3 AMAP1A/MAP1B light chain 3 AMAP1ALC3MAP1BLC3Map1lc3aMicrotubule associated proteins 1A/1B light chain 3Microtubule-associated protein 1 light chain 3 alphaMicrotubule-associated proteins 1A/1B light chain 3AMLP3A_HUMAN
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
MAP1LC3A protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. A coloured control band can be seen at 15 kDa (green). The protein-specific band is blue.
References for MAP1LC3A protein (Tagged-His Tag) (ab92050)
ab92050 has not yet been referenced specifically in any publications.