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Overview

  • Product nameMAP1LC3A protein (Tagged-His Tag)See all MAP1LC3A proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 4-121 (118 amino acids) of Human MAP1LC3A with an N-terminal His Tag; Predicted MWt 15 kDa (UniProt Q9H492).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 108 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQ LPVLDKTKF LVPDHVNMSELVKIIRRRLQLNPTQAFFL LVNQHSMVSVSTPIADIYEQ EKDEDGFLYMVYASQETF GF
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92050 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ATG8EAutophagy-related protein LC3 AAutophagy-related ubiquitin-like modifier LC3 A
        LC3LC3AMAP1 light chain 3 like protein 1MAP1 light chain 3-like protein 1MAP1A/1B light chain 3 AMAP1A/MAP1B LC3 AMAP1A/MAP1B light chain 3 AMAP1ALC3MAP1BLC3Map1lc3aMicrotubule associated proteins 1A/1B light chain 3Microtubule-associated protein 1 light chain 3 alphaMicrotubule-associated proteins 1A/1B light chain 3AMLP3A_HUMAN
      see all
  • FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificityMost abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the MAP1 LC3 family.
  • Post-translational
    modifications    The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.

    • Target information above from: UniProt accession Q9H492 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    MAP1LC3A protein (Tagged-His Tag) images

    • SDS-PAGE - MAP1LC3A protein (Tagged-His Tag) (ab92050)
      SDS-PAGE - MAP1LC3A protein (Tagged-His Tag) (ab92050)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. A coloured control band can be seen at 15 kDa (green). The protein-specific band is blue.

    References for MAP1LC3A protein (Tagged-His Tag) (ab92050)

    ab92050 has not yet been referenced specifically in any publications.

    Publishing research using ab92050 ? Please let us know so that we can cite the reference in this datasheet

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