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Anti-MAP2 antibody [HM-2] - Neuronal Marker
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Mouse monoclonal [HM-2] to MAP2 - Neuronal Marker
Monoclonal Anti-MAP2 does not react with tubulin of other microtubule associated proteins. This antibody recognizes all forms of MAP2 (namely MAP2a, b and c) in human, rat, mouse, bovine, chicken, and quail.
IHC-Fr, ICC/IF, IHC-FrFl, WB, IP, IHC-P, IHC-FoFrmore details
Reacts with
Mouse, Rat, Chicken, Cow, Human, Quail
Rat brain microtubule associated proteins (MAPs).
Rat brain extract.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration information loading...
Protein A purified
Purified from ascites
Monoclonal
HM-2
IgG1
Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267)
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Our Abpromise guarantee covers the use of ab11267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
ICC/IF: 1/500(Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .)
IHC-FrFl: Use at an assay dependent concentration.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 280 kDa.((see Farah et al). )
IP: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-FoFr: 1/500((see recommended protocol).)
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Contains 3 Tau/MAP repeats.
MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP11137
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267)
![Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267)](/ps/datasheet/Images/11/ab11267/ab11267_1.jpg)
ab11267 staining rat brain tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in TritonX100 prior to blocking with 3% BSA for 1 hour. The rpimary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® conjugated goat anti-mouse was used as the secondary antibody.
The image shows a cross section through the rat hipocampal CA1 area at magnification 200x . The anti-MAP2 staining is clearly visible in dendrites of pyramidal cells.
This image is courtesy of an Abreview submitted by Dr Grazyna Niewiadomska
This product has been referenced in:
See all 16 publications for this product
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![Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267)](/ps/datasheet/Images/11/ab11267/ab11267_1.jpg)
ab11267 staining rat brain tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in TritonX100 prior to blocking with 3% BSA for 1 hour. The rpimary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® conjugated goat anti-mouse was used as the secondary antibody.
The image shows a cross section through the rat hipocampal CA1 area at magnification 200x . The anti-MAP2 staining is clearly visible in dendrites of pyramidal cells.
This image is courtesy of an Abreview submitted by Dr Grazyna Niewiadomska
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