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Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267)
![Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267)](/ps/datasheet/images/ab11267_1.jpg)
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Product Name
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MAP2 antibody [HM-2] - Neuronal Marker
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See all MAP2 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [HM-2] to MAP2 - Neuronal Marker
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Immunogen
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Rat brain microtubule associated proteins (MAPs).
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Reacts with
(species key)
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Hu, Ms, Rat, Chk, Cow, Ql
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Specificity
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Monoclonal Anti-MAP2 does not react with tubulin of other microtubule associated proteins.
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC/IF: 1/500. Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) . IHC-Fo: 1/500 (see recommended protocol). IHC-P: 1/50 (PMID 19136970) or use at an assay dependent dilution on Bouin fixed tissue (see recommended protocol). IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 µg/ml (see Farah et al). Detects a band of approximately 280 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat brain extract.
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Cellular localization
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Cytoplasmic. Cytoskeleton.
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Research areas
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Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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Relevance
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MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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HM-2
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 15mM Sodium Azide Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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Purified from ascites
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Form
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Liquid
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Concentration
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Batch dependent within range: 2.10 - 2.30 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAP2 antibody [HM-2] - Neuronal Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAP2 antibody [HM-2] - Neuronal Marker images, references, products related to ab11267 and other tools.
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MAP2 antibody [HM-2] - Neuronal Marker images:
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Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267)
ab11267 staining rat brain tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in TritonX100 prior to blocking with 3% BSA for 1 hour. The rpimary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 594 conjugated goat anti-mouse was used as the secondary antibody. The image shows a cross section through the rat hipocampal CA1 area at magnification 200x . The anti-MAP2 staining is clearly visible in dendrites of pyramidal cells. This image is courtesy of an Abreview submitted by Dr Grazyna Niewiadomska
See Abreview
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References for MAP2 antibody [HM-2] - Neuronal Marker (ab11267)
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This product has been used in: (two most recent references)
Carvalho AF et al. Cannabinoid modulation of limbic forebrain noradrenergic circuitry. Eur J Neurosci : (2010). IHC (PFA fixed); Rat. PubMed: 20074224
Regad T et al. The tumor suppressor Pml regulates cell fate in the developing neocortex. Nat Neurosci 12:132-40 (2009). ICC/IF, IHC-P; Mouse. PubMed: 19136970
See all 16 publication references for this product.
If you publish research using ab11267 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MAP2
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MAP2 antibody [HM-2] - Neuronal Marker - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"