![Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267) image](#ab11267_1.jpg)
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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Overview
Product name
Anti-MAP2 antibody [HM-2] - Neuronal Marker
See all MAP2 products (18) ...
Description
Mouse monoclonal [HM-2] to MAP2 - Neuronal Marker
Specificity
Monoclonal Anti-MAP2 does not react with tubulin of other microtubule associated proteins. This antibody recognizes all forms of MAP2 (namely MAP2a, b and c) in human, rat, mouse, bovine, chicken, and quail.
Tested applications
IHC-Fr, ICC/IF, IHC-FrFl, WB, IP, IHC-P, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Cow, Human, Quail
Immunogen
Rat brain microtubule associated proteins (MAPs).
Positive control
Rat brain extract.
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Purified from ascites
Clonality
Monoclonal
Clone number
HM-2
Isotype
IgG1
Research areas
Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267)
![Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267) image](/ps/datasheet/Images/11/ab11267/ab11267_1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
ICC/IF: 1/500(Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .)
IHC-FrFl: Use at an assay dependent concentration.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 280 kDa.((see Farah et al). )
IP: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-FoFr: 1/500((see recommended protocol).)
Target
Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Sequence similarities
Contains 3 Tau/MAP repeats.
Post-translational
modifications
MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP11137
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686I2148 antibody
- Dendrite specific MAP antibody
- DKFZp686I2148 antibody
see all
Database links
- Entrez Gene: 281294 Cow
- Entrez Gene: 4133 Human
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 368281 Human
see all
Anti-MAP2 antibody [HM-2] - Neuronal Marker images:
Immunohistochemistry (Frozen sections) - MAP2 antibody [HM-2] - Neuronal Marker (ab11267)
ab11267 staining rat brain tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in TritonX100 prior to blocking with 3% BSA for 1 hour. The rpimary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® conjugated goat anti-mouse was used as the secondary antibody.
The image shows a cross section through the rat hipocampal CA1 area at magnification 200x . The anti-MAP2 staining is clearly visible in dendrites of pyramidal cells.
This image is courtesy of an Abreview submitted by Dr Grazyna Niewiadomska
References for Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)
This product has been referenced in:
- Bailey JAet al. Rivastigmine Lowers Aß and Increases sAPPa Levels, Which Parallel Elevated Synaptic Markers and Metabolic Activity in Degenerating Primary Rat Neurons. PLoS One 6:e21954 (2011). ICC/IF; Rat.Read more (PubMed: 21799757) »
- Carvalho AFet al. Cannabinoid modulation of limbic forebrain noradrenergic circuitry. Eur J Neurosci : (2010). IHC (PFA fixed); Rat.Read more (PubMed: 20074224) »
See all 16 publications for this product
Publishing research using ab11267? Please let us know so that we can cite the reference in this datasheet
![MAP2 antibody [HM-2] - Neuronal Marker for IHC-Fr in Rat (11267)](/ps/Reviews/Images/ab9191_44937.jpg)
![MAP2 antibody [HM-2] - Neuronal Marker for Immunohistochemistry - Free Floating in Mouse (11267)](/ps/datasheet/images/11/ab11267/MAP2-Primary-antibodies-ab11267-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"