MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

Product Name 

MAP2 antibody [HM-2] - Neuronal Marker

Product type 

Primary antibodies

Description 

Mouse monoclonal [HM-2] to MAP2 - Neuronal Marker

Immunogen 

Rat brain microtubule associated proteins (MAPs).

Reacts with 
(species key)

Hu, Ms, Rat, Chk, Cow, Ql

Specificity 

Monoclonal Anti-MAP2 does not react with tubulin of other microtubule associated proteins.

Tested applications 
(see key)

ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/500. Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .
IHC-Fo: 1/500 (see recommended protocol).
IHC-P: 1/50 (PMID 19136970) or use at an assay dependent dilution on Bouin fixed tissue (see recommended protocol).
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml (see Farah et al). Detects a band of approximately 280 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Rat brain extract.

Cellular localization 

Cytoplasmic. Cytoskeleton.

Research areas 

Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker

Relevance 

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

HM-2

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites

Form 

Liquid

Concentration 

Batch dependent within range: 2.10 - 2.30 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.

See below for MAP2 antibody [HM-2] - Neuronal Marker images, references, products related to ab11267 and other tools.

MAP2 antibody [HM-2] - Neuronal Marker images:

References for MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

  This product has been used in: (two most recent references)

Carvalho AF et al. Cannabinoid modulation of limbic forebrain noradrenergic circuitry. Eur J Neurosci : (2010). IHC (PFA fixed); Rat. PubMed: 20074224

Regad T et al. The tumor suppressor Pml regulates cell fate in the developing neocortex. Nat Neurosci 12:132-40 (2009). ICC/IF, IHC-P; Mouse. PubMed: 19136970

See all 16 publication references for this product.


If you publish research using ab11267 please let us know so that we can cite the reference on this datasheet.

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