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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Anti-MAP2 antibody - Neuronal Marker (ab32454)
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Proteins and Peptides
ab32453
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab32454? Please let us know so that we can cite the reference in this datasheet
ab32454 has been referenced in 3 publications.
- Yang M et al. Precursor of Brain-derived Neurotrophic Factor (proBDNF) Forms a Complex with Huntingtin-associated Protein-1 (HAP1) and Sortilin That Modulates proBDNF Trafficking, Degradation, and Processing. J Biol Chem 286:16272-84 (2011). ICC/IF; Mouse. PubMed: 21357693
- Lecanu L et al. Caprospinol reduces amyloid deposits and improves cognitive function in a rat model of Alzheimer's disease. Neuroscience 165:427-35 (2010). IHC; Rat. PubMed: 19850110
- Stoyanova II et al. Time-dependent changes in ghrelin-immunoreactivity in dissociated neuronal cultures of the newborn rat neocortex. Regul Pept 158:86-90 (2009). IHC-FoFr; Rat. PubMed: 19540881
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - MAP2 antibody (ab32454)
ICC/IF image of ab32454 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab32454, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAP2 antibody - Neuronal Marker (ab32454)
IHC image of MAP2 staining in human cerebral cortex FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32454, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - MAP2 antibody - Neuronal Marker (ab32454)
All lanes : Anti-MAP2 antibody - Neuronal Marker (ab32454) at 1 µg/ml
Lane 1 :
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 70, 199, 280 kDa
Observed band size : 260,280 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,199 kDa,65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
MAP2 antibody - Neuronal Marker for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Rat (32454)
Anti-MAP2 antibody - Neuronal Marker for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Cat (32454)
MAP2 antibody for IHC-Fr in Mouse (32454)
MAP2 antibody - Neuronal Marker for Immunocytochemistry in Human (32454)
MAP2 antibody - Neuronal Marker for Immunocytochemistry/ Immunofluorescence in Human (32454)
MAP2 antibody - Neuronal Marker for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (32454)
MAP2 antibody for ICC/IF in Mouse (32454)
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