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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Overview
Product name
Anti-MAP2 antibody - Neuronal Marker
See all MAP2 products (18) ...
Description
Chicken polyclonal to MAP2 - Neuronal Marker
Tested applications
IHC-Fr, IHC-FoFr, IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Cynomolgus Monkey
Immunogen
Cow brain MAP 2 purified from the material that pellets with GTP polymerized tubulin (see references).
Positive control
ICC: cultured rat cortical neurons
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.032% Sodium azide
Constituent: PBS
Concentration
Concentration information loading...
Purification notes
Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.
Clonality
Polyclonal
Isotype
IgY
Research areas
Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: 1/500(PubMed: 18684835)
IHC-P: Use at an assay dependent concentration.
WB: 1/100000(This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.)
ICC/IF: 1/10000
Target
Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Sequence similarities
Contains 3 Tau/MAP repeats.
Post-translational
modifications
MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP11137
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686I2148 antibody
- Dendrite specific MAP antibody
- DKFZp686I2148 antibody
see all
Database links
- Entrez Gene: 281294 Cow
- Entrez Gene: 4133 Human
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 368281 Human
see all
Anti-MAP2 antibody - Neuronal Marker images:
Immunohistochemistry (PFA perfusion fixed frozen sections) - MAP2 antibody - Neuronal Marker (ab5392)
ab5392 at 1/5000 staining PFA fixed free floating sections of mouse brain. An AlexaFluor® 633 conjugated goat anti-chicken antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - MAP2 antibody - Neuronal Marker (ab5392)
ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
Immunofluorescence - MAP2 antibody - Neuronal Marker (ab5392)
Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
Immunocytochemistry/ Immunofluorescence - MAP2 antibody - Neuronal Marker (ab5392)
ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-MAP2 antibody - Neuronal Marker (ab5392)
This product has been referenced in:
- Ripley Bet al. Regulation of synaptic stability by AMPA receptor reverse signaling. Proc Natl Acad Sci U S A 108:367-72 (2011). ICC/IF; Rat.Read more (PubMed: 21173224) »
- Laguna Goya Ret al. Human fetal neural precursor cells can up-regulate MHC class I and class II expression and elicit CD4 and CD8 T cell proliferation. Neurobiol Dis 41:407-14 (2011). ICC/IF; Human.Read more (PubMed: 20955796) »
See all 23 publications for this product
Publishing research using ab5392? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"