MAP2 antibody (ab24640)

Product Name 

MAP2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MAP2

Immunogen 

Brain MAP2 (Cow)

Reacts with 
(species key)

Hu, Ms, Rat, Mamm

Tested applications 
(see key)

EM, ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
EM: Use at an assay dependent dilution (PMID 19020041).
ICC/IF: 1/200 (PMID 18403755).
IF: 1/1000.
IHC-P: 1/200 (PMID 18403755).
IHC-FrFl: Use at an assay dependent dilution (PMID 20154682).
WB: 1/1000.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic. Cytoskeleton.

Research areas 

Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker

Relevance 

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.

The antibody will stain neuronal cell bodies and dendritic processes, and will not stain glial cells or axons. It is the best marker to distinguish dendrites from axons in the brain.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

antiserum

Purification notes 

ab24640 was electrophoretically purified from heat stable MAP fraction.

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References for MAP2 antibody (ab24640)

  This product has been used in: (two most recent references)

Anderson D et al. Regulation of neuronal activity by Cav3-Kv4 channel signaling complexes. Nat Neurosci 13:333-7 (2010). IHC-FrFl; Rat. PubMed: 20154682

Liu Y et al. Novel Interaction of the Dopamine D2 Receptor and the Ca2+-Binding Protein S100B: Role in D2 Receptor Function. Mol Pharmacol : (2008). ICC/IF; Rat. PubMed: 18445708

See all 6 publication references for this product.


If you publish research using ab24640 please let us know so that we can cite the reference on this datasheet.

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