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Products:Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules
Overview
Product name
Anti-MAP2a + MAP2b antibody [1.B.642] (Biotin)
See all MAP2a + MAP2b products (3) ...
Description
Mouse monoclonal [1.B.642] to MAP2a + MAP2b (Biotin)
Conjugation
Biotin
Specificity
ab63518 reacts with high molecular weight isoforms of MAP2 (MAP2a and MAP2b), but not with the low molecular weight isoform MAP2c.
Tested applications
IHC-Fr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Cow, Human
Immunogen
300kDa MAP2 protein from bovine brain
Positive control
Dendrites or cell bodies of neurons.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
1.B.642
Isotype
IgG1
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Neuronal
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IHC-Fr: 1/500IHC-Fr: 1/500
WB: Use at an assay dependent dilution.
Target
Relevance
MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.
Cellular localization
Cytoplasm, cytoskeleton
Alternative names
- Microtubule associated protein 2 antibody
- MAP 2 antibody
- MAP 2a antibody
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Database links
- Entrez Gene: 424001 Chicken
- Entrez Gene: 281294 Cow
- Entrez Gene: 4133 Human
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P20357 Mouse
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References for Anti-MAP2a + MAP2b antibody [1.B.642] (Biotin) (ab63518)
ab63518 has not yet been referenced specifically in any publications.
Publishing research using ab63518? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"