MAP2a + MAP2b antibody [AP20] (FITC) (ab24279)

Product Name 

MAP2a + MAP2b antibody [AP20] (FITC)

Product type 

Primary antibodies

Description 

Mouse monoclonal [AP20] to MAP2a + MAP2b (FITC)

Immunogen 

The details of the immunogen for this antibody are not available.

Reacts with 
(species key)

Hu, Ms, Rat, Chk

Specificity 

ab24279 reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). It reacts with dendrites and cell bodies of neurons.

Tested applications 
(see key)

IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-Fr: 1/500. WB: Use at an assay dependent dilution. Detects a band of approximately 300 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasm, cytoskeleton

Research areas 

Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Neuronal
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules

Relevance 

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

AP20

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1.0% BSA, 0.01M PBS, 2.0mM EDTA. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C in the dark. Do not freeze.

References for MAP2a + MAP2b antibody [AP20] (FITC) (ab24279)

  This clone has been used in:

Reubinoff BE et al. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 18:399-404 (2000). PubMed: 10748519
If you publish research using ab24279 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding MAP2a + MAP2b antibody [AP20] (FITC)

Customer FAQs regarding MAP2a + MAP2b antibody [AP20] (FITC)

Protocols for MAP2a + MAP2b antibody [AP20] (FITC)

Price and availability of products related to MAP2a + MAP2b antibody [AP20] (FITC)

MAP2a + MAP2b antibody [AP20] (FITC) - related products: