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Immunocytochemistry/ Immunofluorescence - MAP2a + MAP2b antibody [AP20] - Neuronal Marker (ab3096)
![Immunocytochemistry/ Immunofluorescence - MAP2a + MAP2b antibody [AP20] - Neuronal Marker (ab3096)](/ps/datasheet/images/ab3096_2.jpeg)
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Immunohistochemistry (Frozen sections) - MAP2a + MAP2b antibody [AP20] - Neuronal Marker (ab3096)
![Immunohistochemistry (Frozen sections) - MAP2a + MAP2b antibody [AP20] - Neuronal Marker (ab3096)](/ps/datasheet/images/3/ab3096/MAP2a-MAP2b-Primary-antibodies-ab3096-4.jpg)
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Product Name
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MAP2a + MAP2b antibody [AP20] - Neuronal Marker
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See all MAP2a + MAP2b antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [AP20] to MAP2a + MAP2b - Neuronal Marker
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Immunogen
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Bovine brain MAP 2.
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Reacts with
(species key)
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Hu, Ms, Rat, Chk, Cow, Ql, Xl
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Specificity
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This antibody is highly specific to MAP 2 a + b and shows no cross-reaction with MAP 1, MAP 5, tubulin, or tau.
It reacts with dendrites and cell bodies of neurons but not with the neuronal processes.
It does not react with the low molecular weight (70kDa) form (2c) of MAP 2.
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Epitope
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Amino acids 997 - 1332.
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution (see review).
IHC-P: Use at a concentration of 1 - 2 µg/ml (30mins at RT). Perform heat mediated antigen retrieval before commencing with IHC staining protocol (10mM citrate buffer pH 6.0, for 10-20 min followed by cooling at RT for 20 mins).
IHC-Fr: 1/200.
WB: Use at a concentration of 1 - 2 µg/ml ( 2 hours at RT). Detects a band of approximately 280 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human glioblastoma T98G cells or brain.
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Cellular localization
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Cytoplasm, cytoskeleton
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Neuronal
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules
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Relevance
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MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.
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MAP2a + MAP2b antibody [AP20] - Neuronal Marker (ab3096) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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AP20
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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Notes
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This antibody is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer’s.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAP2a + MAP2b antibody [AP20] - Neuronal Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAP2a + MAP2b antibody [AP20] - Neuronal Marker images, references, products related to ab3096 and other tools.
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MAP2a + MAP2b antibody [AP20] - Neuronal Marker images:
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 Immunocytochemistry/ Immunofluorescence - MAP2a + MAP2b antibody [AP20] - Neuronal Marker (ab3096)
This image was taken with a 5x objective and is from the CA3 region of rat hippocampus. It is possible to see cell soma slightly stained and the dendrites are also well stained. Only pyramidal cells are labelled here and there are no processes from interneurons. This is not a confocal reconstruction. This image is courtesy of an Abreview submitted by Dr Luis Craveiro
See Abreview
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Immunohistochemistry (Frozen sections) - MAP2a + MAP2b antibody [AP20] - Neuronal Marker (ab3096)
ab3096 at a 1/200 dilution staining MAP2a + MAP2b in whole rat embryo tissue sections by Immunohistochemistry (frozen sections) incubated at 25°C for 16 hours. PFA fixed. Blocked using 5% serum for 1 hour at 25°C. Secondary used at 1/200 polyclonal Goat anti-mouse conjugated to Alexa Fluor 546. This image is courtesy of an anonymous abreview.
See Abreview
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References for MAP2a + MAP2b antibody [AP20] - Neuronal Marker (ab3096)
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This product has been used in: (two most recent references)
Meuth SG et al. Cytotoxic CD8+ T cell-neuron interactions: perforin-dependent electrical silencing precedes but is not causally linked to neuronal cell death. J Neurosci 29:15397-409 (2009). ICC/IF; Mouse. PubMed: 20007464
Craveiro LM et al. Neutralization of the membrane protein Nogo-A enhances growth and reactive sprouting in established organotypic hippocampal slice cultures. Eur J Neurosci 28:1808-24 (2008). ICC/IF; Rat. PubMed: 18973596
See all 7 publication references for this product.
If you publish research using ab3096 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MAP2a + MAP2b
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MAP2a + MAP2b antibody [AP20] - Neuronal Marker - more information
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Customer reviews (feedback) regarding MAP2a + MAP2b antibody [AP20] - Neuronal Marker |
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Price and availability of products related to MAP2a + MAP2b antibody [AP20] - Neuronal Marker |
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MAP2a + MAP2b antibody [AP20] - Neuronal Marker - related products:
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MAP2a + MAP2b antibody [AP20] - Neuronal Marker - other tools:
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Contact Abcam with a Technical Enquiry about MAP2a + MAP2b antibody [AP20] - Neuronal Marker |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Frozen sections) - MAP2a + MAP2b antibody [AP20] - Neuronal Marker (ab3096)](/index.html?datasheet=3096#MAP2a-MAP2b-Primary-antibodies-ab3096-4.jpg)
