MAP2a + MAP2b antibody [MT-07] (ab36447)

Product Name 

MAP2a + MAP2b antibody [MT-07]

Product type 

Primary antibodies

Description 

Mouse monoclonal [MT-07] to MAP2a + MAP2b

Immunogen 

Full length native protein (purified)
Microtubule protein enriched for kinesin

Reacts with 
(species key)

Hu, Ms, Pig

Specificity 

ab36447 recognizes Microtubule associated protein 2ab (MAP2ab).

Tested applications 
(see key)

ELISA, ICC, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: Use at a concentration of 5 µg/ml.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 - 5 µg/ml. Predicted molecular weight: 200 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Porcine brain, Mouse brain, human neuroblastoma.

Cellular localization 

Cytoplasm, cytoskeleton

Research areas 

Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Neuronal
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules

Relevance 

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

MT-07

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from hybridoma culture supernatant: > 95% (by SDS-PAGE).

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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