Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910)
Key features and details
- Rabbit monoclonal [EP630Y] to MAP4K1/HPK1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-MAP4K1/HPK1 antibody [EP630Y]
See all MAP4K1/HPK1 primary antibodies -
Description
Rabbit monoclonal [EP630Y] to MAP4K1/HPK1 -
Host species
Rabbit -
Specificity
ab33910 recognises hematopoietic progenitor kinase 1 (HPK1).
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji and Daudi (human Burkitt's lymphoma) whole cell lysates. IHC-P: Human liver tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.09% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP630Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 97 kDa.
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IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 97 kDa. |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. -
Tissue specificity
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 11184 Human
- Omim: 601983 Human
- SwissProt: Q92918 Human
- Unigene: 95424 Human
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Alternative names
- Hematopoietic progenitor kinase 1 antibody
- Hematopoietic progenitor kinase antibody
- HPK1 antibody
see all
Images
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All lanes : Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910) at 1/5000 dilution
Lane 1 : Daudi (human Burkitt's lymphoma) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 97 kDaBlocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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ab33910 staining MAP4K1/HPK1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab33910 has been referenced in 5 publications.
- Lim S et al. Identification of the kinase STK25 as an upstream activator of LATS signaling. Nat Commun 10:1547 (2019). PubMed: 30948712
- Chen X et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). PubMed: 31293967
- Chen Y et al. Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes. Cell Death Differ 26:1346-1364 (2019). PubMed: 30341421
- Li Y et al. Ubiquitin-specific protease 4 inhibits breast cancer cell growth through the upregulation of PDCD4. Int J Mol Med 38:803-11 (2016). Human . PubMed: 27430936
- Palmer RD et al. Pediatric malignant germ cell tumors show characteristic transcriptome profiles. Cancer Res 68:4239-47 (2008). IHC-P ; Human . PubMed: 18519683