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Overview

  • Product nameMAPK10 protein (Active)See all MAPK10 proteins and peptides ...
  • Protein descriptionRecombinant full length Mouse MAPK10 (amino acids 1-464) with an N terminal proprietary tag; MWt 71 kDa (UniProt Q61831).
  • Uniprot accessionQ61831
  • Molecular weight71kDa inclusive of tags
  • Protein length464 amino acids
  • Expression hostBaculovirus infected sf9 cells

    • Properties

  • Purity> 80 % by SDS-PAGE
  • Purification notesPurity determined to be >80% by densitometry.
  • Biological activitySpecific Activity: 35 nmol/min/mg
  • E.C. Number2.7.11.24
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferpH: 7.50
    Constituents: 0.79% Tris HCl, 25% Glycerol, 0.004% DTT, 0.003% EDTA, 0.31% Glutathione, 0.002% PMSF, 0.88% Sodium chloride
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab119734 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        c Jun kinase 3c-Jun N-terminal kinase 3cJun N terminal kinase 3
        FLJ12099FLJ33785JNK3JNK3 alpha protein kinaseJNK3AMAP kinaseMAP kinase 10MAP kinase p49 3F12MAPK 10Mapk10MGC50974mitogen activated protein kinase 10Mitogen-activated protein kinase 10MK10_HUMANp493F12p54bSAPKPRKM10protein kinase mitogen activated 10SAPK1bStress activated protein kinase 1bstress activated protein kinase betaStress activated protein kinase JNK3Stress-activated protein kinase JNK3
      see all
  • FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity.
  • Tissue specificitySpecific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
  • Involvement in diseaseDefects in MAPK10 are a cause of epileptic encephalopathy Lennox-Gastaut type (EELG) [MIM:606369]. Epileptic encephalopathies of the Lennox-Gastaut group are childhood epileptic disorders characterized by severe psychomotor delay and seizures. Note=A chromosomal aberration involving MAPK10 has been found in a single patient. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications    Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P53779 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    MAPK10 protein (Active) images

    • Functional Studies - MAPK10 protein (ab119734)
      Functional Studies - MAPK10 protein (ab119734)
      The specific activity of ab119734 was determined to be 35 nmol/min/mg.
    • SDS-PAGE - MAPK10 protein (ab119734)
      SDS-PAGE - MAPK10 protein (ab119734)
      SDS-PAGE analysis of ab119374.

    References for MAPK10 protein (Active) (ab119734)

    ab119734 has not yet been referenced specifically in any publications.

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