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RabMAbs (1497-1)

Anti-MAPKAP Kinase 2 antibody [E341] (ab32567)

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Overview

  • Product nameAnti-MAPKAP Kinase 2 antibody [E341]See all MAPKAP Kinase 2 primary antibodies ...
  • Description
    Rabbit monoclonal [E341] to MAPKAP Kinase 2
  • Specificityab32567 recognises MAPKAP Kinase-2.
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near C-terminus of human MAPKKAP Kinase-2.

  • Positive controlHela cell lysate.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab32567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
  • Application notesIP: 1/200.
    WB: 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

    Is unsuitable for FACS, ICC or IHC.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
    • Tissue specificityExpressed in all tissues examined.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications      Phosphorylated and activated by MAP kinase.

      • Target information above from: UniProt accession P49137 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        MAP Kinase Activated Protein Kinase 2 antibodyMAP kinase-activated protein kinase 2 antibodyMAPK activated protein kinase 2 antibody
        MAPK-activated protein kinase 2 antibodyMAPK2_HUMAN antibodyMAPKAP K2 antibodyMAPKAP kinase 2 antibodyMAPKAPK 2 antibodyMAPKAPK-2 antibodyMAPKAPK2 antibodyMitogen Activated Protein Kinase Activated Protein Kinase 2 antibodyMK 2 antibodyMK2 antibody
      see all

    Anti-MAPKAP Kinase 2 antibody [E341] images

    • Western blot - MAPKAP Kinase 2 antibody [E341] (ab32567)
      Western blot - MAPKAP Kinase 2 antibody [E341] (ab32567)
      Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/1000 dilution + Hela cell lysate

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • Western blot - Anti-MAPKAP Kinase 2 antibody [E341] (ab32567)
      Western blot - Anti-MAPKAP Kinase 2 antibody [E341] (ab32567)
      Lanes 1 - 2 : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
      Lanes 3 - 4 : Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/500 dilution

      Lane 1 : UV treated HeLa cells
      Lane 2 : UV treated HeLa cells + p38 inhibitor
      Lane 3 : UV treated HeLa cells
      Lane 4 : UV treated HeLa cells + p38 inhibitor

      Secondary
      IRDye® 680 LICOR goat anti-rabbit.

      Performed under reducing conditions.

      Predicted band size : 46 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible cross reactivity).

      Exposure time : 1 minute

      See Abreview

    References for Anti-MAPKAP Kinase 2 antibody [E341] (ab32567)

    ab32567 has not yet been referenced specifically in any publications.

    Publishing research using ab32567 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-MAPKAP Kinase 2 antibody [E341]

     Excellent
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cells)
    Loading amount 35 µg
    Specification HeLa cells
    Treatment UV treated samples (20 J dose)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Aug 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"