Anti-MAPKAP Kinase 2 antibody [E341] (ab32567)

Overview

  • Product nameAnti-MAPKAP Kinase 2 antibody [E341]
    See all MAPKAP Kinase 2 primary antibodies
  • Description
    Rabbit monoclonal [E341] to MAPKAP Kinase 2
  • Specificityab32567 recognises MAPKAP Kinase-2.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near C-terminus of human MAPKKAP Kinase-2.

  • Positive control
    • Hela cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IP 1/200.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC.
  • Target

    • FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
    • Tissue specificityExpressed in all tissues examined.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated and activated by MAP kinase.
    • Information by UniProt
    • Database links
    • Alternative names
      • MAP kinase activated protein Kinase 2 antibody
      • MAP kinase-activated protein kinase 2 antibody
      • MAPK activated protein kinase 2 antibody
      • MAPK-activated protein kinase 2 antibody
      • MAPK2_HUMAN antibody
      • MAPKAP K2 antibody
      • MAPKAP kinase 2 antibody
      • MAPKAPK 2 antibody
      • MAPKAPK-2 antibody
      • MAPKAPK2 antibody
      • Mitogen activated protein kinase activated protein kinase 2 antibody
      • MK 2 antibody
      • MK2 antibody
      see all

    Anti-MAPKAP Kinase 2 antibody [E341] images



    • Predicted band size : 46 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/1000 dilution + Hela cell lysate

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • Lanes 1 - 2 : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
      Lanes 3 - 4 : Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/500 dilution

      Lane 1 : UV treated HeLa cells
      Lane 2 : UV treated HeLa cells + p38 inhibitor
      Lane 3 : UV treated HeLa cells
      Lane 4 : UV treated HeLa cells + p38 inhibitor

      Secondary
      IRDye® 680 LICOR goat anti-rabbit.

      Performed under reducing conditions.

      Predicted band size : 46 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible cross reactivity).

      Exposure time : 1 minute
      ab32567 recognise total MK2 (lane 3,4). A control for specificity is shown as UV treated HeLa + p38 inhibitor (lane 2,4). While the intensity of pT334 band is greatly reduced (lane 2), total MK2 remains unaltered (lane 4).

      See Abreview

    References for Anti-MAPKAP Kinase 2 antibody [E341] (ab32567)

    ab32567 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cells)
    Loading amount 35 µg
    Specification HeLa cells
    Treatment UV treated samples (20 J dose)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Aug 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"