You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-MAPKAP Kinase 2 antibody (ab2311)

Submit an Abreview Q&A (4)

Overview

  • Product nameAnti-MAPKAP Kinase 2 antibodySee all MAPKAP Kinase 2 primary antibodies ...
  • Description
    Rabbit polyclonal to MAPKAP Kinase 2
  • SpecificityTwo isoforms are produced due to alternative splicing of the same gene which differ in their C-terminals. This antibody recognizes both 43 and 60 kDa isoforms, corresponding to the apparent molecular mass of MAPKAPK-2 on SDS-PAGE immunoblots. These 43 kDa and 60 kDa bands can be specifically inhibited by the relevant peptide.
  • Tested applicationsIP, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

Properties

Applications

Our Abpromise guarantee covers the use of ab2311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP: Use a concentration of 4 µg/ml.
WB WB: Use a concentration of 4 µg/ml.

Target

  • FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
  • Tissue specificityExpressed in all tissues examined.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylated and activated by MAP kinase.

    • Target information above from: UniProt accession P49137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      MAP Kinase Activated Protein Kinase 2 antibodyMAP kinase-activated protein kinase 2 antibodyMAPK activated protein kinase 2 antibody
      MAPK-activated protein kinase 2 antibodyMAPK2_HUMAN antibodyMAPKAP K2 antibodyMAPKAP kinase 2 antibodyMAPKAPK 2 antibodyMAPKAPK-2 antibodyMAPKAPK2 antibodyMitogen Activated Protein Kinase Activated Protein Kinase 2 antibodyMK 2 antibodyMK2 antibody
    see all

References for Anti-MAPKAP Kinase 2 antibody (ab2311)

ab2311 has not yet been referenced specifically in any publications.

Publishing research using ab2311 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab2311 Submit a Question for ab2311

Displaying 1 - 4 of 4 results for Abreviews and Q&A

10
Votes: Highest First

I contact you because I would be interested to purchase the anti-MAPKAP 2 antibody (ref ab2311). However, I need to be sure that the immunogenic peptide used to raise this antibody is included in the first 317 amino acids of MAPKAP2. Indeed, we need to de...

Read More

Thank you for your enquiry. The immunogen used to raise this Ab is a synthetic peptide mapping to the C-terminus of human MAPKAP2. The full immunogenic sequence is deemed to be commercially sensitive by the originator. We only supply one other Ab to t...

Read More

I have used your MAPKAP Kinase 2 (C-term) antibody, cat# ab2311, for western blotting at the concentration that is recommended(4 ug/ml) and at lower concentrations(1 ug/ml,0.1 ug/ml). There were many bands detected in the neutrophil protein Western so I m...

Read More

Thank you so much for your patience. For both blots your customer has done, they are definitely having a problem with non-specific bands. They have decreased the concentration of the primary, but have they checked that these bands are not coming from th...

Read More

I am considering purchasing your polyclonal antibody to MAPKAP Kinase 2 (ab2311), but I need to know at least some information about the immunogen to know if it is appropriate for my needs. Does the immunogen include at least some portion of aa 1-320 (...

Read More

The immunogen is peptide arround a.a. 350 of human MAPKAPK-2. It does not include portion of aa 1-320.

I would like to know whether this antibody has been tested for use in immunocytochemistry or histochemistry.

All the tested applications for each of our products are specified on the datasheets. This antibody has not yet been tested in IHC. However, if you decide to go ahead and purchase it please let us know how you get on and in return we will forward a reward...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"