Overview

  • Product nameAnti-MAPKAP Kinase 2 antibodySee all MAPKAP Kinase 2 primary antibodies ...
  • Description
    Rabbit polyclonal to MAPKAP Kinase 2
  • Specificityab63574 detects endogenous levels of total MAPKAP Kinase 2 protein.
  • Tested applicationsELISA, IHC-P, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non phosphopeptide derived from human MAPKAP Kinase 2 around the phosphorylation site of threonine 334 (P-Q-TP-P-L).

  • Positive control
    • IHC-P: Human breast carcinoma tissue WB: HeLa cell extracts treated with Ca2+ (40nM, 30mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab63574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.

Target

  • FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
  • Tissue specificityExpressed in all tissues examined.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated and activated by MAP kinase.
  • Target information above from: UniProt accession P49137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • MAP kinase activated protein Kinase 2 antibody
    • MAP kinase-activated protein kinase 2 antibody
    • MAPK activated protein kinase 2 antibody
    • MAPK-activated protein kinase 2 antibody
    • MAPK2_HUMAN antibody
    • MAPKAP K2 antibody
    • MAPKAP kinase 2 antibody
    • MAPKAPK 2 antibody
    • MAPKAPK-2 antibody
    • MAPKAPK2 antibody
    • Mitogen activated protein kinase activated protein kinase 2 antibody
    • MK 2 antibody
    • MK2 antibody
    see all

Anti-MAPKAP Kinase 2 antibody images

  • ab63574, at 1/50 dilution, staining MAPKAP Kinase 2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
  • All lanes : Anti-MAPKAP Kinase 2 antibody (ab63574) at 1/500 dilution

    Lane 1 : HeLa cell extracts treated with Ca2+ (40nM, 30mins)
    Lane 2 : HeLa cell extracts treated with Ca2+ (40nM, 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 25 kDa,30 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-MAPKAP Kinase 2 antibody (ab63574) at 1/500 dilution

    Lane 1 : Human MAPKAP Kinase 2 full length protein (ab79910) at 0.01 µg
    Lane 2 : Human MAPKAP Kinase 2 full length protein (ab79910) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

References for Anti-MAPKAP Kinase 2 antibody (ab63574)

ab63574 has not yet been referenced specifically in any publications.

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