Anti-MAPKAP Kinase 2 antibody (ab63574)

Overview

• Product nameAnti-MAPKAP Kinase 2 antibodySee all MAPKAP Kinase 2 primary antibodies ...
• Description
  Rabbit polyclonal to MAPKAP Kinase 2
• Specificityab63574 detects endogenous levels of total MAPKAP Kinase 2 protein.
• Tested applicationsELISA, IHC-P, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic non phosphopeptide derived from human MAPKAP Kinase 2 around the phosphorylation site of threonine 334 (P-Q-T^P-P-L).

• Positive controlIHC-P: Human breast carcinoma tissue WB: HeLa cell extracts treated with Ca²⁺ (40nM, 30mins).

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab63574 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Cardiovascular
  • Blood
  • Other

Applications

Target

• FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
• Tissue specificityExpressed in all tissues examined.
• Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylated and activated by MAP kinase.

Target information above from: UniProt accession P49137 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9261 Human
  • Entrez Gene: 17164 Mouse
  • Entrez Gene: 289014 Rat
  • Omim: 602006 Human
  • SwissProt: P49137 Human
  • SwissProt: P49138 Mouse
  • Unigene: 643566 Human
  • Unigene: 713747 Human

  • Unigene: 221235 Mouse
  • Unigene: 6276 Rat

  see all

• Alternative names
  MAP kinase activated protein Kinase 2 antibodyMAP kinase-activated protein kinase 2 antibodyMAPK activated protein kinase 2 antibody

  MAPK-activated protein kinase 2 antibodyMAPK2_HUMAN antibodyMAPKAP K2 antibodyMAPKAP kinase 2 antibodyMAPKAPK 2 antibodyMAPKAPK-2 antibodyMAPKAPK2 antibodyMitogen activated protein kinase activated protein kinase 2 antibodyMK 2 antibodyMK2 antibody

  see all

Anti-MAPKAP Kinase 2 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAPKAP Kinase 2 antibody (ab63574)
  ab63574, at 1/50 dilution, staining MAPKAP Kinase 2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
• 

  Western blot - MAPKAP Kinase 2 antibody (ab63574)
  All lanes : Anti-MAPKAP Kinase 2 antibody (ab63574) at 1/500 dilution
  
  Lane 1 : HeLa cell extracts treated with Ca²⁺ (40nM, 30mins)
  Lane 2 : HeLa cell extracts treated with Ca²⁺ (40nM, 30mins) with immunising peptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
  Additional bands at : 25 kDa,30 kDa. We are unsure as to the identity of these extra bands.
• 

  Western blot - Anti-MAPKAP Kinase 2 antibody (ab63574)
  All lanes : Anti-MAPKAP Kinase 2 antibody (ab63574) at 1/500 dilution
  
  Lane 1 : MAPKAP Kinase 2 protein (Tagged) (ab79910) at 0.01 µg
  Lane 2 : MAPKAP Kinase 2 protein (Tagged) (ab79910) at 0.001 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 90 seconds