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RabMAbs (1983-1)

Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)

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Overview

  • Product nameAnti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y]See all MAPKAP Kinase 2 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR992Y] to MAPKAP Kinase 2 (phospho T334)
  • Tested applicationsWB, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Phosphorylated synthetic peptide corresponding to residues near C terminus of human MAPKAP Kinase 2.

  • Positive controlWB: HeLa cell lysate IHC-P: Human breast carcinoma tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab51018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. We strongly recommend that customers perform an antigen retrieval step.
    WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).

    Is unsuitable for Flow Cyt or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
    • Tissue specificityExpressed in all tissues examined.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications      Phosphorylated and activated by MAP kinase.

      • Target information above from: UniProt accession P49137 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          MAP kinase activated protein Kinase 2 antibodyMAP kinase-activated protein kinase 2 antibodyMAPK activated protein kinase 2 antibody
          MAPK-activated protein kinase 2 antibodyMAPK2_HUMAN antibodyMAPKAP K2 antibodyMAPKAP kinase 2 antibodyMAPKAPK 2 antibodyMAPKAPK-2 antibodyMAPKAPK2 antibodyMitogen activated protein kinase activated protein kinase 2 antibodyMK 2 antibodyMK2 antibody
        see all

      Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] images

      • Western blot - MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)
        Western blot - MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)
        All lanes : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution

        Lane 1 : HeLa cell lysate, untreated
        Lane 2 : HeLa cell lysate treated with anisomycin

        Lysates/proteins at 10 µg per lane.

        Secondary
        Goat anti-rabbit HRP labeled at 1/2000 dilution

        Predicted band size : 46 kDa
        Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      • Immunohistochemistry (Paraffin-embedded sections) - MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)
        Immunohistochemistry (Paraffin-embedded sections) - MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)
        Ab51018 (1/100) staining human MAPKAP kinase 2 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
      • Western blot - Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)
        Western blot - Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)
        All lanes : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)

        Lane 1 : HEK293 cells transfected with GFP-MK2
        Lane 2 : HEK293 cells transfected with GFP-MK2 and treated 1h with p38 inhibitor
        Lane 3 : HEK293 cells transfected with GFP-T334A MK2


        Predicted band size : 46 kDa

        See Abreview

      References for Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)

      This product has been referenced in:
      • Felix RS  et al. SAGE analysis highlights the importance of p53csv, ddx5, mapkapk2 and ranbp2 to multiple myeloma tumorigenesis. Cancer Lett 278:41-8 (2009). IHC-P ; Human . Read more (PubMed: 19171422) »

      See 1 Publication for this product

      Publishing research using ab51018 ? Please let us know so that we can cite the reference in this datasheet

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      Western blot abreview for Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y]

       Good
      Abreviews
      Application Western blot
      Sample Human Cell lysate - whole cell (HEK293 cells)
      Loading amount 35 µg
      Specification HEK293 cells
      Treatment Irradiated with 20 Joules and collected 1h later
      Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
      Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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      Submitted May 06 2012

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"