RabMAb

Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)

Overview

  • Product nameAnti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y]
    See all MAPKAP Kinase 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR992Y] to MAPKAP Kinase 2 (phospho T334)
  • SpecificityThe immunogen sequence used to produce ab51018 is 90% identical to the corresponding region of MAPKAP Kinase 3, therefore ab51018 may cross react with human MAPKAP Kinase 3 but this has not been confirmed.
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Phosphorylated synthetic peptide corresponding to residues near C terminus of human MAPKAP Kinase 2.

  • Positive control
    • WB: HeLa cell lysate IHC-P: Human breast carcinoma tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab51018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. We strongly recommend that customers perform an antigen retrieval step.
    WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).

    Is unsuitable for Flow Cyt or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
    • Tissue specificityExpressed in all tissues examined.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated and activated by MAP kinase.
    • Information by UniProt
    • Database links
    • Alternative names
      • MAP kinase activated protein Kinase 2 antibody
      • MAP kinase-activated protein kinase 2 antibody
      • MAPK activated protein kinase 2 antibody
      • MAPK-activated protein kinase 2 antibody
      • MAPK2_HUMAN antibody
      • MAPKAP K2 antibody
      • MAPKAP kinase 2 antibody
      • MAPKAPK 2 antibody
      • MAPKAPK-2 antibody
      • MAPKAPK2 antibody
      • Mitogen activated protein kinase activated protein kinase 2 antibody
      • MK 2 antibody
      • MK2 antibody
      see all

    Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] images

    • All lanes : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with anisomycin

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • Ab51018 (1/100) staining human MAPKAP kinase 2 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • All lanes : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)

      Lane 1 : HEK293 cells transfected with GFP-MK2
      Lane 2 : HEK293 cells transfected with GFP-MK2 and treated 1h with p38 inhibitor
      Lane 3 : HEK293 cells transfected with GFP-T334A MK2


      Predicted band size : 46 kDa

      See Abreview

    References for Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)

    This product has been referenced in:
    • Tate CM  et al. LY2228820 dimesylate, a selective inhibitor of p38 mitogen-activated protein kinase, reduces angiogenic endothelial cord formation in vitro and in vivo. J Biol Chem 288:6743-53 (2013). WB . Read more (PubMed: 23335506) »
    • Felix RS  et al. SAGE analysis highlights the importance of p53csv, ddx5, mapkapk2 and ranbp2 to multiple myeloma tumorigenesis. Cancer Lett 278:41-8 (2009). IHC-P ; Human . Read more (PubMed: 19171422) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293 cells)
    Loading amount 35 µg
    Specification HEK293 cells
    Treatment Irradiated with 20 Joules and collected 1h later
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"