RabMAb

Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)

Overview

  • Product nameAnti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y]See all MAPKAP Kinase 2 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR992Y] to MAPKAP Kinase 2 (phospho T334)
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Phosphorylated synthetic peptide corresponding to residues near C terminus of human MAPKAP Kinase 2.

  • Positive control
    • WB: HeLa cell lysate IHC-P: Human breast carcinoma tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab51018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. We strongly recommend that customers perform an antigen retrieval step.
    WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).

    Is unsuitable for Flow Cyt or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
    • Tissue specificityExpressed in all tissues examined.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated and activated by MAP kinase.
    • Information by UniProt
    • Database links
    • Alternative names
      • MAP kinase activated protein Kinase 2 antibody
      • MAP kinase-activated protein kinase 2 antibody
      • MAPK activated protein kinase 2 antibody
      • MAPK-activated protein kinase 2 antibody
      • MAPK2_HUMAN antibody
      • MAPKAP K2 antibody
      • MAPKAP kinase 2 antibody
      • MAPKAPK 2 antibody
      • MAPKAPK-2 antibody
      • MAPKAPK2 antibody
      • Mitogen activated protein kinase activated protein kinase 2 antibody
      • MK 2 antibody
      • MK2 antibody
      see all

    Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] images

    • All lanes : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with anisomycin

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • Ab51018 (1/100) staining human MAPKAP kinase 2 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • All lanes : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)

      Lane 1 : HEK293 cells transfected with GFP-MK2
      Lane 2 : HEK293 cells transfected with GFP-MK2 and treated 1h with p38 inhibitor
      Lane 3 : HEK293 cells transfected with GFP-T334A MK2


      Predicted band size : 46 kDa

      See Abreview

    References for Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018)

    This product has been referenced in:
    • Tate CM  et al. LY2228820 dimesylate, a selective inhibitor of p38 mitogen-activated protein kinase, reduces angiogenic endothelial cord formation in vitro and in vivo. J Biol Chem 288:6743-53 (2013). WB . Read more (PubMed: 23335506) »
    • Felix RS  et al. SAGE analysis highlights the importance of p53csv, ddx5, mapkapk2 and ranbp2 to multiple myeloma tumorigenesis. Cancer Lett 278:41-8 (2009). IHC-P ; Human . Read more (PubMed: 19171422) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293 cells)
    Loading amount 35 µg
    Specification HEK293 cells
    Treatment Irradiated with 20 Joules and collected 1h later
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"