Anti-MAPKAP Kinase 2 (phospho T334) antibody (ab63378)

Overview

• Product nameAnti-MAPKAP Kinase 2 (phospho T334) antibodySee all MAPKAP Kinase 2 primary antibodies ...
• Description
  Rabbit polyclonal to MAPKAP Kinase 2 (phospho T334)
• SpecificityDetects endogenous levels of MAPKAP Kinase 2 only when phosphorylated at threonine 334 (human) and threonine 320 (mouse and rat).
• Tested applicationsICC/IF, WB, IHC-P, ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic phosphopeptide from around the phosphorylation site of threonine 334 (PQT^PPL) of human MAPKAP Kinase 2

• Positive controlHuman breast carcinoma tissue NIH/3T3 cell extract

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Cardiovascular
  • Blood
  • Other

Applications

Target

• FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
• Tissue specificityExpressed in all tissues examined.
• Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylated and activated by MAP kinase.

Target information above from: UniProt accession P49137 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9261 Human
  • Entrez Gene: 17164 Mouse
  • Entrez Gene: 289014 Rat
  • Omim: 602006 Human
  • SwissProt: P49137 Human
  • SwissProt: P49138 Mouse
  • Unigene: 643566 Human
  • Unigene: 713747 Human

  • Unigene: 221235 Mouse
  • Unigene: 6276 Rat

  see all

• Alternative names
  MAP kinase activated protein Kinase 2 antibodyMAP kinase-activated protein kinase 2 antibodyMAPK activated protein kinase 2 antibody

  MAPK-activated protein kinase 2 antibodyMAPK2_HUMAN antibodyMAPKAP K2 antibodyMAPKAP kinase 2 antibodyMAPKAPK 2 antibodyMAPKAPK-2 antibodyMAPKAPK2 antibodyMitogen activated protein kinase activated protein kinase 2 antibodyMK 2 antibodyMK2 antibody

  see all

Anti-MAPKAP Kinase 2 (phospho T334) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAPKAP Kinase 2 (phospho T334) antibody (ab63378)
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelled with ab63378 at 1/50 dilution. Right hand panel was treated with immunising phosphopeptide.
• 

  Western blot - MAPKAP Kinase 2 (phospho T334) antibody (ab63378)
  All lanes : Anti-MAPKAP Kinase 2 (phospho T334) antibody (ab63378) at 1/500 dilution
  
  Lane 1 : NIH/3T3 cell extract
  Lane 2 : NIH/3T3 cell extract with immunising phosphopeptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
  Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence-MAPKAP Kinase 2 (phospho T334) antibody(ab63378)
  ICC/IF image of ab63378 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63378, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.