Overview
Product nameMAPKAP Kinase 3 protein (T7 Tag)See all MAPKAP Kinase 3 proteins and peptides ...
Protein descriptionRecombinant full length (human) protein, with a T7 tag at the N terminus.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
FormLiquid
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.002% Sodium Azide
Constituents: 0.1% Triton-X-100, 10mM Tris, 10mM DTT, pH 8.0
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab40524
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Application notesThis peptide can be used with studies using ab155178.
Protein info
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Alternative names
3PKanti MAPKAPK3Chromosome 3p kinase
EC 2.7.11.1MAP kinase-activated protein kinase 3MAPK activated protein kinase 3MAPK-activated protein kinase 3MAPK3_HUMANMAPKAPMAPKAP kinase 3MAPKAP-K3MAPKAP3MAPKAPK 3MAPKAPK-3Mapkapk3MAPKAPK3 antibodyMitogen activated protein kinase activated protein kinaseMitogen activated protein kinase activated protein kinase 3MK-3
see all
FunctionStress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
Tissue specificityWidely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Post-translational
modificationsPhosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313.
Cellular localizationNucleus. Cytoplasm. Predominantly located in the nucleus, when activated it translocates to the cytoplasm.
References for MAPKAP Kinase 3 protein (T7 Tag) (ab40524)
ab40524
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
