Anti-MAPKAPK2/3 antibody (ab82543)

Overview

• Product nameAnti-MAPKAPK2/3 antibody
• Description
  Rabbit polyclonal to MAPKAPK2/3
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide, corresponding to amino acids 332-336 of Human MAPKAPK2

• Positive controlRecombinant proteins

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, pH 7.4
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

Applications

Target

• RelevanceMAPKAPK2 (MAP kinase activated protein kinase 2, MAPKAP Kinase 2), also known as p45 hsp27 kinase, is a 45-54 kDa serine/threonine protein kinase that contains a proline rich sequence and two putative SH3 binding sites. MAPKAP Kinase 2 is activated in response to stress, IL1 and TNF, possibly catalyzed by p38/Hog dependent phosphorylation. One of the major substrates of MAPKAP Kinase 2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. MAPKAP2 is implicated in several disorders including ischemic brain injury and heart failure and has been shown to be important in regulating stress resistance and the production of TNF alpha. MAPKAPK3 functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation
• Cellular localizationCytoplasmic and Nuclear
• Database links
  • Entrez Gene: 9261 Human
  • Entrez Gene: 7867 Human
  • Omim: 602006 Human
  • Omim: 602130 Human
  • SwissProt: P49137 Human
  • SwissProt: Q16644 Human

• Alternative names
  3PK antibodyAA960234 antibodyMAP kinase activated protein kinase 2 antibody

  MAP kinase activated protein kinase 3 antibodyMAPK activated protein kinase 2 antibodyMAPK activated protein kinase 3 antibodyMAPKAP kinase 2 antibodyMAPKAP kinase 3 antibodyMK2 antibodyMK3 antibodyOTTHUMP00000034531 antibodyRps6kc1 antibody

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Anti-MAPKAPK2/3 antibody images

• 

  Western blot - MAPKAPK2/3 antibody (ab82543)
  All lanes : Anti-MAPKAPK2/3 antibody (ab82543) at 1/1000 dilution
  
  Lane 1 : Human recombinant MAPKAPK2 with proprietary tag
  Lane 2 : human recombinant MAPKAPK3 with proprietary tag
  
  Lysates/proteins at 0.02 µg per lane.
  
  
  Predicted band size : 43 , 45 kDa
  Observed band size : 65,70 kDa (why is the actual band size different from the predicted?)