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Anti-MARK1 antibody (ab60202)

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Overview

Product name

Anti-MARK1 antibody
See all MARK1 products (3) ...

Description

Rabbit polyclonal to MARK1

Tested applications

IHC-P, ELISA, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic peptide from the N terminal region (human), conjugated to KLH.

Positive control

IHC-P: Human brain tissue. WB: P7 mouse whole brain lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

ab60202 was purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MARK

  • Western blot - MARK1 antibody (ab60202)Western blot - MARK1 antibody (ab60202) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MARK1 antibody (ab60202)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MARK1 antibody (ab60202) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab60202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/10 - 1/50.

    IHC-P: 1/10 - 1/50.

  • ELISA

     ELISA: 1/1000

    ELISA: 1/1000

  • ShowHide1 Image

    WB

     WB: 1/200 - 1/500.Detect...Read more →

    WB: 1/200 - 1/500.Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).

Target

Relevance

MARK1 (MAP/microtubule affinity-regulating kinase 1) belongs to the MARK family of serine/threonine kinases. MARK family protein kinases phosphorylate microtubule-associated proteins and may play a role in cytoskeletal stability.

Cellular localization

Cytoplasmic, cytoskeleton. Note=Appears to localize to an intracellular network.

Alternative names

  • KIAA1477 antibody
  • MAP/microtubule affinity regulating kinase 1 antibody
  • MARK 1 antibody
  • MARK antibody
  • MGC126512 antibody
  • MGC126513 antibody
  • Serine/threonine protein kinase MARK1 antibody
see all

Database links

Anti-MARK1 antibody images:

  Western blot - MARK1 antibody (ab60202)

Western blot - MARK1 antibody (ab60202)

Anti-MARK1 antibody (ab60202) at 1/250 dilution + P7 mouse whole brain lysate at 60 µg

Predicted band size : 89 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MARK1 antibody (ab60202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MARK1 antibody (ab60202)

ab60202, at 1/50 dilution, staining MARK1 in formalin fixed, paraffin-embedded human brain tissue by immunohistochemistry. ab60202 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References for Anti-MARK1 antibody (ab60202)

ab60202 has not yet been referenced specifically in any publications.

Publishing research using ab60202? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"