Overview
- Product nameAnti-MARK2 antibody [5C12D2]See all MARK2 primary antibodies ...
- DescriptionMouse monoclonal [5C12D2] to MARK2
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
- Immunogen
Synthetic peptide: PDYKDPRRTELMVSMGYTRE conjugated to KLH, corresponding to amino acids 288-307 of Human MARK2
- Positive controlHeLa cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, Ascites -
Concentration information loading... - PurityAscites
- Clonality Monoclonal
- Clone number5C12D2
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab77698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000. Predicted molecular weight: 87 kDa. |
Target
- FunctionRole in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
- Tissue specificityHigh levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
- Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. - Cellular localizationCell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
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Database links
- Entrez Gene: 535197 Cow
- Entrez Gene: 483769 Dog
- Entrez Gene: 2011 Human
- Entrez Gene: 2011 Human
- Entrez Gene: 2011 Human
- Entrez Gene: 13728 Mouse
- Entrez Gene: 60328 Rat
- Omim: 600526 Human
- SwissProt: Q7KZI7 Human
- SwissProt: Q7KZI7 Human
- SwissProt: Q7KZI7 Human
- SwissProt: Q05512 Mouse
- SwissProt: O08679 Rat
- Unigene: 567261 Human
- Unigene: 258986 Mouse
- Unigene: 42926 Rat
see all
Target information above from: UniProt accession
Q7KZI7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- EC 2.7.11.1 antibodyELKL motif kinase 1 antibodyELKL motif kinase antibody
- EMK-1 antibodyEMK1 antibodyMAP/microtubule affinity regulating kinase 2 antibodyMAP/microtubule affinity-regulating kinase 2 antibodyMark2 antibodyMARK2_HUMAN antibodyMGC99619 antibodyPAR 1 antibodyPar 1b antibodyPAR1 homolog antibodyPar1b antibodySer/Thr protein kinase PAR 1B antibodySerine/threonine protein kinase EMK antibodySerine/threonine protein kinase MARK2 antibodySerine/threonine-protein kinase MARK2 antibody
see all
Anti-MARK2 antibody [5C12D2] images
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![Western blot - MARK2 antibody [5C12D2] (ab77698)](http://a.abcam.com/ps/datasheet/Images/77/ab77698/ab77698_1.jpg)
Western blot - MARK2 antibody [5C12D2] (ab77698)Anti-MARK2 antibody [5C12D2] (ab77698) at 1/1000 dilution + Hela cell lysate
Secondary
Goat anti-mouse HRP conjugate
Predicted band size : 87 kDa
Observed band size : 87 kDa
References for Anti-MARK2 antibody [5C12D2] (ab77698)
ab77698 has not yet been referenced specifically in any publications.
