Overview

  • Product name
  • Description
    Goat polyclonal to MARK2
  • Specificity
    Expected to recognize both reported isoforms b and c (NP_004945.3 and NP_001034557.1).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    C-QNGKDSTAPQR

    , corresponding to internal sequence amino acids 499-509 of Human MARK2 (NP_001034557.1)

  • Positive control
    • Human Brain (Cerebellum) lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/16000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 77 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
    • Tissue specificity
      High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
      Contains 1 KA1 (kinase-associated) domain.
      Contains 1 protein kinase domain.
      Contains 1 UBA domain.
    • Cellular localization
      Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ELKL motif kinase 1 antibody
      • ELKL motif kinase antibody
      • EMK-1 antibody
      • EMK1 antibody
      • MAP/microtubule affinity regulating kinase 2 antibody
      • MAP/microtubule affinity-regulating kinase 2 antibody
      • Mark2 antibody
      • MARK2_HUMAN antibody
      • MGC99619 antibody
      • PAR 1 antibody
      • Par 1b antibody
      • PAR1 homolog antibody
      • Par1b antibody
      • Ser/Thr protein kinase PAR 1B antibody
      • Serine/threonine protein kinase EMK antibody
      • Serine/threonine protein kinase MARK2 antibody
      • Serine/threonine-protein kinase MARK2 antibody
      see all

    Images

    References

    This product has been referenced in:
    • Lázaro-Diéguez F  et al. Par1b links lumen polarity with LGN-NuMA positioning for distinct epithelial cell division phenotypes. J Cell Biol 203:251-64 (2013). Read more (PubMed: 24165937) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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