Overview

  • Product nameAnti-MARK2 (phospho T595) antibody
    See all MARK2 primary antibodies
  • Description
    Rabbit polyclonal to MARK2 (phospho T595)
  • SpecificityReactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4.
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Cow, Dog, Human
  • Immunogen

    Synthetic peptide:

    VSSRSTFHAGQLR

    , corresponding to amino acids 591-603 of Human MARK2

  • Positive control
    • HEK293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab34751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.
WB 1/500 - 1/3000. Detects a band of approximately 82 kDa (predicted molecular weight: 87 kDa).

Target

  • FunctionRole in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
  • Tissue specificityHigh levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Cellular localizationCell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.11.1 antibody
    • ELKL motif kinase 1 antibody
    • ELKL motif kinase antibody
    • EMK-1 antibody
    • EMK1 antibody
    • MAP/microtubule affinity regulating kinase 2 antibody
    • MAP/microtubule affinity-regulating kinase 2 antibody
    • Mark2 antibody
    • MARK2_HUMAN antibody
    • MGC99619 antibody
    • PAR 1 antibody
    • Par 1b antibody
    • PAR1 homolog antibody
    • Par1b antibody
    • Ser/Thr protein kinase PAR 1B antibody
    • Serine/threonine protein kinase EMK antibody
    • Serine/threonine protein kinase MARK2 antibody
    • Serine/threonine-protein kinase MARK2 antibody
    see all

Anti-MARK2 (phospho T595) antibody images

  • All lanes : Anti-MARK2 (phospho T595) antibody (ab34751) at 1/500 dilution

    Lane 1 : MW markers
    Lane 2 : untransfected HEK293 cell lysate
    Lane 3 : HEK293 cells transfected with pCMV-3×Flag- MARK2 plus pCMV-Flag-PKC-zeta
    Lane 4 : HEK293 cells transfected with pCMV-3×Flag-MARK2 only


    Predicted band size : 87 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)

References for Anti-MARK2 (phospho T595) antibody (ab34751)

This product has been referenced in:
  • Wu-Zhang AX  et al. Cellular Pharmacology of Protein Kinase M? (PKM?) Contrasts with Its in Vitro Profile: IMPLICATIONS FOR PKM? AS A MEDIATOR OF MEMORY. J Biol Chem 287:12879-85 (2012). WB ; Rat . Read more (PubMed: 22378786) »

See 1 Publication for this product

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