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Anti-MARK2 (phospho T595) antibody (ab34751)

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Overview

Product name

Anti-MARK2 (phospho T595) antibody
See all MARK2 products (6) ...

Description

Rabbit polyclonal to MARK2 (phospho T595)

Specificity

Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4.

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Rat

Predicted to work with

Mouse, Cow, Dog, Human

Immunogen

Synthetic peptide: VSSRSTFHAGQLR, corresponding to amino acids 591-603 of Human MARK2

VSSRSTFHAGQLR

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HEK293 cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MARK

  • Western blot - MARK2 (phospho T595) antibody (ab34751)Western blot - MARK2 (phospho T595) antibody (ab34751) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab34751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.

Tissue specificity

High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.

Cellular localization

Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.

Target information above from: UniProt accessionQ7KZI7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EC 2.7.11.1 antibody
  • ELKL motif kinase 1 antibody
  • ELKL motif kinase antibody
  • EMK-1 antibody
  • EMK1 antibody
  • MAP/microtubule affinity regulating kinase 2 antibody
  • MAP/microtubule affinity-regulating kinase 2 antibody
  • Mark2 antibody
  • MARK2_HUMAN antibody
  • MGC99619 antibody
  • PAR 1 antibody
  • Par 1b antibody
  • PAR1 homolog antibody
  • Par1b antibody
  • Ser/Thr protein kinase PAR 1B antibody
  • Serine/threonine protein kinase EMK antibody
  • Serine/threonine protein kinase MARK2 antibody
  • Serine/threonine-protein kinase MARK2 antibody
see all

Database links

see all

Anti-MARK2 (phospho T595) antibody images:

  Western blot - MARK2 (phospho T595) antibody (ab34751)

Western blot - MARK2 (phospho T595) antibody (ab34751)

All lanes : Anti-MARK2 (phospho T595) antibody (ab34751) at 1/500 dilution

Lane 1 : MW markers
Lane 2 : untransfected HEK293 cell lysate
Lane 3 : HEK293 cells transfected with pCMV-3×Flag- MARK2 plus pCMV-Flag-PKC-zeta
Lane 4 : HEK293 cells transfected with pCMV-3×Flag-MARK2 only


Predicted band size : 87 kDa
Observed band size : 82 kDa (why is the actual band size different from the predicted?)

References for Anti-MARK2 (phospho T595) antibody (ab34751)

This product has been referenced in:

  • Wu-Zhang AX  et al. Cellular Pharmacology of Protein Kinase M? (PKM?) Contrasts with Its in Vitro Profile: IMPLICATIONS FOR PKM? AS A MEDIATOR OF MEMORY. J Biol Chem 287:12879-85 (2012). WB; Rat.Read more (PubMed: 22378786) »

See 1 publication for this product

Publishing research using ab34751? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"