Anti-MARK2 (phospho T595) antibody (ab34751)

Overview

• Product nameAnti-MARK2 (phospho T595) antibodySee all MARK2 primary antibodies ...
• Description
  Rabbit polyclonal to MARK2 (phospho T595)
• SpecificityReactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4.
• Tested applicationsELISA, WB more details
• Species reactivity
  Reacts with: Rat
  Predicted to work with: Mouse, Cow, Dog, Human
• Immunogen

  Synthetic peptide: VSSRSTFHAGQLR, corresponding to amino acids 591-603 of Human MARK2

  Run BLAST with Run BLAST with
• Positive controlHEK293 cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe product was affinity purified from monospecific antiserum by immunoaffinity purification.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microtubules
  • MT Associated Proteins
  • MARK

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

Applications

Target

• FunctionRole in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
• Tissue specificityHigh levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
• Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
  Contains 1 KA1 (kinase-associated) domain.
  Contains 1 protein kinase domain.
  Contains 1 UBA domain.
• Cellular localizationCell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.

Target information above from: UniProt accession Q7KZI7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 535197 Cow
  • Entrez Gene: 483769 Dog
  • Entrez Gene: 2011 Human
  • Entrez Gene: 2011 Human
  • Entrez Gene: 2011 Human
  • Entrez Gene: 13728 Mouse
  • Entrez Gene: 60328 Rat
  • Omim: 600526 Human

  • SwissProt: Q7KZI7 Human
  • SwissProt: Q7KZI7 Human
  • SwissProt: Q7KZI7 Human
  • SwissProt: Q05512 Mouse
  • SwissProt: O08679 Rat
  • Unigene: 567261 Human
  • Unigene: 258986 Mouse
  • Unigene: 42926 Rat

  see all

• Alternative names
  EC 2.7.11.1 antibodyELKL motif kinase 1 antibodyELKL motif kinase antibody

  EMK-1 antibodyEMK1 antibodyMAP/microtubule affinity regulating kinase 2 antibodyMAP/microtubule affinity-regulating kinase 2 antibodyMark2 antibodyMARK2_HUMAN antibodyMGC99619 antibodyPAR 1 antibodyPar 1b antibodyPAR1 homolog antibodyPar1b antibodySer/Thr protein kinase PAR 1B antibodySerine/threonine protein kinase EMK antibodySerine/threonine protein kinase MARK2 antibodySerine/threonine-protein kinase MARK2 antibody

  see all

Anti-MARK2 (phospho T595) antibody images

• 

  Western blot - MARK2 (phospho T595) antibody (ab34751)
  All lanes : Anti-MARK2 (phospho T595) antibody (ab34751) at 1/500 dilution
  
  Lane 1 : MW markers
  Lane 2 : untransfected HEK293 cell lysate
  Lane 3 : HEK293 cells transfected with pCMV-3×Flag- MARK2 plus pCMV-Flag-PKC-zeta
  Lane 4 : HEK293 cells transfected with pCMV-3×Flag-MARK2 only
  
  
  Predicted band size : 87 kDa
  Observed band size : 82 kDa (why is the actual band size different from the predicted?)