SpecificityReactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
1/500 - 1/3000. Detects a band of approximately 82 kDa (predicted molecular weight: 87 kDa).
FunctionRole in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
Tissue specificityHigh levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily. Contains 1 KA1 (kinase-associated) domain. Contains 1 protein kinase domain. Contains 1 UBA domain.
Cellular localizationCell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
Western blot - Anti-MARK2 (phospho T595) antibody (ab34751)
All lanes : Anti-MARK2 (phospho T595) antibody (ab34751) at 1/500 dilution
Lane 1 : MW markers Lane 2 : untransfected HEK293 cell lysate Lane 3 : HEK293 cells transfected with pCMV-3×Flag-
MARK2 plus pCMV-Flag-PKC-zeta Lane 4 : HEK293 cells transfected with pCMV-3×Flag-MARK2 only
Wu-Zhang AX et al. Cellular Pharmacology of Protein Kinase M? (PKM?) Contrasts with Its in Vitro Profile: IMPLICATIONS FOR PKM? AS A MEDIATOR OF MEMORY. J Biol Chem287:12879-85 (2012).
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