Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab5262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
WB
  • Application notesPeptide ELISA: Antibody detection limit dilution 1:128,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 75 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificityUbiquitous. Isoform 2 is brain-specific.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
      Contains 1 KA1 (kinase-associated) domain.
      Contains 1 protein kinase domain.
      Contains 1 UBA domain.
    • Post-translational
      modifications
      Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
    • Target information above from: UniProt accession Q96L34 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • FLJ90097 antibody
      • KIAA1860 antibody
      • MAP / microtubule affinity regulating kinase 4 antibody
      • MAP / microtubule affinity regulating kinase 4L antibody
      • MAP/microtubule affinity regulating kinase 4 antibody
      • MAP/microtubule affinity regulating kinase like 1 antibody
      • MAP/microtubule affinity-regulating kinase 4 antibody
      • MAP/microtubule affinity-regulating kinase-like 1 antibody
      • MARK 4 antibody
      • MARK 4L antibody
      • MARK L1 antibody
      • MARK4 antibody
      • MARK4 serine / threonine protein kinase antibody
      • MARK4 serine/threonine protein kinase antibody
      • MARK4_HUMAN antibody
      • MARK4S antibody
      • MARKL 1 antibody
      • MARKL1 antibody
      • Nbla00650 antibody
      • Putative protein product of Nbla00650 antibody
      see all

    Anti-MARK4 antibody images

    References for Anti-MARK4 antibody (ab5262)

    ab5262 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. The immunogen used was the synthetic peptide IKRKPPPGCSDSPGV, corresponding to C terminal amino acids 674-688 of Human MARK4. Please note that ab5262 is a "Fast-Track" antibody (like your boot camp antibodies!). Fast-...

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    Thank you for your enquiry and your interest in our products. The only ELISA we have done tests binding of the antibody to the peptide used for immunization. As the datasheet says our antibody detection limit dilution was found to be 1:32000. I...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"