Overview

  • Product nameAnti-MASPIN antibodySee all MASPIN primary antibodies ...
  • Description
    Rabbit polyclonal to MASPIN
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human MASPIN.

    (Peptide available as ab24350.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Human Prostate Tumor.

Properties

Applications

Our Abpromise guarantee covers the use of ab22354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human MASPIN peptide (ab24350).

Target

  • FunctionTumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificityNormal mammary epithelial cells.
  • Sequence similaritiesBelongs to the serpin family. Ov-serpin subfamily.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • mammary serine proteinase inhibitor antibody
    • Maspin antibody
    • Ovalbumin antibody
    • Peptidase inhibitor 5 antibody
    • PI-5 antibody
    • PI5 antibody
    • Protease inhibitor 5 antibody
    • Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody
    • Serpin B5 antibody
    • serpin peptidase inhibitor antibody
    • Serpin peptidase inhibitor, clade B (ovalbumin), member 5 antibody
    • SERPINB5 antibody
    • SPB5_HUMAN antibody
    • Spi5 antibody
    • Spi7 antibody
    see all

Anti-MASPIN antibody images

  • All lanes : Anti-MASPIN antibody (ab22354) at 1 µg/ml

    Lane 1 : Human Prostate Tumor Tissue Lysate at 20 ug
    Lane 2 : Human Prostate Tumor Tissue Lysate at 20 ug with Human MASPIN peptide (ab24350) at 1 µg/ml

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible cleavage fragment).
  • ab22354 staining MASPIN in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in TRIS-EDTA Buffer pH 9.0 and permeabilized with a wash buffer with Tween. The primary antibody was diluted 1/2000 and incubated with the sample for 30 minutes at 20°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-MASPIN antibody (ab22354)

ab22354 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Wash-Buffer from Dako with Tween
Username

Mr. Rudolf Jung

Verified customer

Submitted May 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"