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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Integrins
Overview
Product name
Anti-MAdCAM1 antibody [MECA-367] (Biotin)
See all MAdCAM1 products (10) ...
Description
Rat monoclonal [MECA-367] to MAdCAM1 (Biotin)
Conjugation
Biotin
Tested applications
Flow Cyt, IHC-Fr, Blocking, IP, WBmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Endothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes.
Epitope
ab25021 reacts with an epitope located in the first domain of MAdCAM1.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
ab25021 blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer’s patch HEV In vivo.
Clonality
Monoclonal
Clone number
MECA-367
Isotype
IgG2a
Light chain type
kappa
Research areas
Neuroscience >> Cell Adhesion Proteins >> Receptors >> Integrins >> Beta Integrins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Integrins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
AP: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
IHC-Fr: Use a concentration of 1 µg/ml
BL: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.
Target
Function
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Tissue specificity
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
Sequence similarities
Contains 2 Ig-like (immunoglobulin-like) domains.
Post-translational
modifications
The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ13477
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Addressin mucosal antibody
- hMAdCAM 1 antibody
- hMAdCAM-1 antibody
see all
Database links
- Entrez Gene: 17123 Mouse
- SwissProt: Q61826 Mouse
- Unigene: 391556 Mouse
References for Anti-MAdCAM1 antibody [MECA-367] (Biotin) (ab25021)
ab25021 has not yet been referenced specifically in any publications.
Publishing research using ab25021? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"