MBD1 antibody [100B272.1.] - ChIP Grade (ab45028)

Product Name 

MBD1 antibody [100B272.1.] - ChIP Grade

Product type 

Primary antibodies

Description 

Mouse monoclonal [100B272.1.] to MBD1 - ChIP Grade

Immunogen 

This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1.
BLAST 'SESEDGAGSPPPYRR' with or

Reacts with 
(species key)

Hu

Specificity 

ab45028 detects MBD1, isoform 1.

Tested applications 
(see key)

ChIP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 15327775).
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding

Relevance 

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

100B272.1.

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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MBD1 antibody [100B272.1.] - ChIP Grade images:

References for MBD1 antibody [100B272.1.] - ChIP Grade (ab45028)

  This product has been used in: (two most recent references)

Sarraf SA & Stancheva I Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Mol Cell 15:595-605 (2004). ChIP; Human. PubMed: 15327775

Reese BE et al. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. Mol Cell Biol 23:3226-36 (2003). PubMed: 12697822
If you publish research using ab45028 please let us know so that we can cite the reference on this datasheet.

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