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Read our guarantee »Anti-MBD1 antibody [100B272.1.] - ChIP Grade
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Mouse monoclonal [100B272.1.] to MBD1 - ChIP Grade
ab45028 detects MBD1, isoform 1.
WB, ChIPmore details
Reacts with
Human
This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1.
SESEDGAGSP PPYRR
HeLa cell lysate.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration information loading...
Protein G purified
Monoclonal
100B272.1.
IgG1
Western blot - MBD1 antibody [100B272.1.] (ab45028)
(enlarge)
Our Abpromise guarantee covers the use of ab45028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution (PMID 15327775).
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
Widely expressed.
Contains 3 CXXC-type zinc fingers.
Contains 1 MBD (methyl-CpG-binding) domain.
The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
Target information above from: UniProt accessionQ9UIS9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - MBD1 antibody [100B272.1.] (ab45028)
![Western blot - MBD1 antibody [100B272.1.] (ab45028)](/ps/datasheet/Images/45/ab45028/ab45028.jpg)
Lane 1 : Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 2 µg/ml
Lane 2 : Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate
Predicted band size : 67 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab45028? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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![Western blot - MBD1 antibody [100B272.1.] (ab45028)](/ps/datasheet/Images/45/ab45028/ab45028.jpg)
Lane 1 : Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 2 µg/ml
Lane 2 : Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate
Predicted band size : 67 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
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