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Western blot - MBD1 antibody [100B272.1.] (ab45028)
![Western blot - MBD1 antibody [100B272.1.] (ab45028)](/ps/datasheet/images/ab45028.jpg)
(enlarge)
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Product Name
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MBD1 antibody [100B272.1.] - ChIP Grade
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See all MBD1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [100B272.1.] to MBD1 - ChIP Grade
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Immunogen
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This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1. BLAST 'SESEDGAGSPPPYRR' with or 
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Reacts with
(species key)
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Hu
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Specificity
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ab45028 detects MBD1, isoform 1.
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Tested applications
(see key)
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ChIP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ChIP: Use at an assay dependent dilution (PMID 15327775). WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
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Relevance
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DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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100B272.1.
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: PBS Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MBD1 antibody [100B272.1.] - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MBD1 antibody [100B272.1.] - ChIP Grade images, references, products related to ab45028 and other tools.
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MBD1 antibody [100B272.1.] - ChIP Grade images:
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Western blot - MBD1 antibody [100B272.1.] (ab45028)
Lane 1 : MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 2 µg/ml Lane 2 : MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 1 µg/ml
Lane 1 : HeLa cell lysate Lane 2 : HeLa cell lysate
Predicted band size : 67 kDa Observed band size : 70 kDa (why is the actual band size different from the predicted?)
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References for MBD1 antibody [100B272.1.] - ChIP Grade (ab45028)
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This product has been used in: (two most recent references)
Sarraf SA & Stancheva I Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Mol Cell 15:595-605 (2004). ChIP; Human. PubMed: 15327775
Reese BE et al. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. Mol Cell Biol 23:3226-36 (2003). PubMed: 12697822
If you publish research using ab45028 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MBD1
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MBD1 antibody [100B272.1.] - ChIP Grade - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"