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Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028)

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Overview

Product name

Anti-MBD1 antibody [100B272.1.] - ChIP Grade
See all MBD1 products (8) ...

Description

Mouse monoclonal [100B272.1.] to MBD1 - ChIP Grade

Specificity

ab45028 detects MBD1, isoform 1.

Tested applications

WB, ChIPmore details

Cross reactivity

Reacts with

Human

Immunogen

This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1.

SESEDGAGSP PPYRR

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa cell lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

100B272.1.

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding

  • Western blot - MBD1 antibody [100B272.1.] (ab45028)Western blot - MBD1 antibody [100B272.1.] (ab45028) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab45028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ChIP: Use at an assay dependent dilution (PMID 15327775).
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

Tissue specificity

Widely expressed.

Sequence similarities

Contains 3 CXXC-type zinc fingers.
Contains 1 MBD (methyl-CpG-binding) domain.

Domain

The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.

Post-translational
modifications

Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
Phosphorylated upon DNA damage, probably by ATM or ATR.

Cellular localization

Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.

Target information above from: UniProt accessionQ9UIS9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CXXC 3 antibody
  • CXXC-type zinc finger protein 3 antibody
  • CXXC3 antibody
  • MBD 1 antibody
  • MBD1 antibody
  • MBD1 antibody
  • MBD1_HUMAN antibody
  • MECP1 COMPLEX antibody
  • Methyl CpG binding domain protein 1 antibody
  • Methyl CpG binding domain protein 1 antibody
  • Methyl CpG binding domain protein 1 isoform PCM1 antibody
  • Methyl CpG binding protein MBD1 antibody
  • Methyl CpG binding protein splice variant 1 antibody
  • Methyl CpG binding protein splice variant 2 antibody
  • Methyl CpG binding protein splice variant 3 antibody
  • Methyl CpG binding protein splice variant 4 antibody
  • Methyl-CpG-binding domain protein 1 antibody
  • Methyl-CpG-binding protein MBD1 antibody
  • PCM 1 antibody
  • PCM 1 antibody
  • PCM1 antibody
  • Protein containing methyl CpG binding domain 1 antibody
  • Protein containing methyl-CpG-binding domain 1 antibody
  • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
  • RFT antibody
  • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
see all

Database links

Anti-MBD1 antibody [100B272.1.] - ChIP Grade images:

  Western blot - MBD1 antibody [100B272.1.] (ab45028)

Western blot - MBD1 antibody [100B272.1.] (ab45028)

Lane 1 : Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 2 µg/ml
Lane 2 : Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 1 µg/ml

Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate


Predicted band size : 67 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028)

This product has been referenced in:

  • Reese BEet al. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. Mol Cell Biol 23:3226-36 (2003).Read more (PubMed: 12697822) »
  • Sarraf SA & Stancheva I Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Mol Cell 15:595-605 (2004). ChIP; Human.Read more (PubMed: 15327775) »

See all 2 publications for this product

Publishing research using ab45028? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"