MBD1 antibody - ChIP Grade (ab3753)

Product Name 

MBD1 antibody - ChIP Grade

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MBD1 - ChIP Grade

Immunogen 

Synthetic peptide: KQEPPDPEEDKEENKDDSAS, corresponding to C terminal amino acids 436-455 of Human MBD1.
BLAST 'KQEPPDPEEDKEENKDDSAS' with or

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Mamm
(Due to sequence homology)

Epitope 

This antibody recognises the C-terminus of MBD 1.

Tested applications 
(see key)

ChIP, ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependant dilution.
IF: Use at an assay dependant dilution.
WB: 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 57.6 kDa). We recommend using nuclear extracts when performing Western blots with this antibody.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MCF 7 cells.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding

Relevance 

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

serum with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for MBD1 antibody - ChIP Grade images, references, products related to ab3753 and other tools.

MBD1 antibody - ChIP Grade images:

References for MBD1 antibody - ChIP Grade (ab3753)

  This product has been used in: (two most recent references)

Bierne H et al. Human BAHD1 promotes heterochromatic gene silencing. Proc Natl Acad Sci U S A 106:13826-31 (2009). WB; Human. PubMed: 19666599

Fu J et al. Promoter regulation of the visinin-like subfamily of neuronal calcium sensor proteins by nuclear respiratory factor-1. J Biol Chem 284:27577-86 (2009). ChIP; Human. PubMed: 19674972

See all 6 publication references for this product.


If you publish research using ab3753 please let us know so that we can cite the reference on this datasheet.

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