Anti-MBD1 antibody - ChIP Grade (ab3753)

Overview

• Product nameAnti-MBD1 antibody - ChIP GradeSee all MBD1 primary antibodies ...
• Description
  Rabbit polyclonal to MBD1 - ChIP Grade
• Tested applicationsWB, ICC/IF, ChIP more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: all Mammals
• Immunogen

  Synthetic peptide: KQEPPDPEEDKEENKDDSAS, corresponding to C terminal amino acids 436-455 of Human MBD1.

  Run BLAST with Run BLAST with
• EpitopeThis antibody recognises the C-terminus of MBD 1.
• Positive controlMCF 7 cells.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferserum with 0.02% sodium azide
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA methylation
  • Methylated DNA Binding

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

Applications

Target

• FunctionTranscriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
• Tissue specificityWidely expressed.
• Sequence similaritiesContains 3 CXXC-type zinc fingers.
  Contains 1 MBD (methyl-CpG-binding) domain.
• DomainThe methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
  The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
  The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
• Post-translational
  modificationsSumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
  Phosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationNucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.

Target information above from: UniProt accession Q9UIS9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4152 Human
  • Entrez Gene: 17190 Mouse
  • Omim: 156535 Human
  • SwissProt: Q9UIS9 Human
  • SwissProt: Q9Z2E2 Mouse
  • Unigene: 405610 Human
  • Unigene: 22522 Mouse

• Alternative names
  CXXC 3 antibodyCXXC-type zinc finger protein 3 antibodyCXXC3 antibody

  MBD 1 antibodyMBD1 antibodyMBD1_HUMAN antibodyMECP1 COMPLEX antibodyMethyl CpG binding domain protein 1 antibodyMethyl CpG binding domain protein 1 antibodyMethyl CpG binding domain protein 1 isoform PCM1 antibodyMethyl CpG binding protein MBD1 antibodyMethyl CpG binding protein splice variant 1 antibodyMethyl CpG binding protein splice variant 2 antibodyMethyl CpG binding protein splice variant 3 antibodyMethyl CpG binding protein splice variant 4 antibodyMethyl-CpG-binding domain protein 1 antibodyMethyl-CpG-binding protein MBD1 antibodyPCM 1 antibodyPCM 1 antibodyPCM1 antibodyProtein containing methyl CpG binding domain 1 antibodyProtein containing methyl-CpG-binding domain 1 antibodyRegulator of fibroblast growth factor 2 (FGF 2) transcription antibodyRFT antibodyThe regulator of fibroblast growth factor 2(FGF 2) transcription antibody

  see all

Anti-MBD1 antibody - ChIP Grade images

• 

  Western blot - MBD1 antibody - ChIP Grade (ab3753)
  
  
  Predicted band size : 57.6 kDa
  
  
  1. Western blot using Rabbit polyclonal to MBD 1 at a 1/1000 dilution MCF 7 cell lysate. The antibody detects a band at approximately 60 kDa.
  
  2. Immunolocalisation of MBD 1 in PAM212 cells.