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Anti-MBD1 antibody - ChIP Grade (ab3753)

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Overview

Product name

Anti-MBD1 antibody - ChIP Grade
See all MBD1 products (8) ...

Description

Rabbit polyclonal to MBD1 - ChIP Grade

Tested applications

WB, IF, ChIPmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

all Mammals

Immunogen

Synthetic peptide: KQEPPDPEEDKEENKDDSAS, corresponding to C terminal amino acids 436-455 of Human MBD1.

KQEPPDPEED KEENKDDSAS

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Epitope

This antibody recognises the C-terminus of MBD 1.

Positive control

MCF 7 cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

serum with 0.02% sodium azide

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding

  • Western blot - MBD1 antibody - ChIP Grade (ab3753)Western blot - MBD1 antibody - ChIP Grade (ab3753) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

Tissue specificity

Widely expressed.

Sequence similarities

Contains 3 CXXC-type zinc fingers.
Contains 1 MBD (methyl-CpG-binding) domain.

Domain

The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.

Post-translational
modifications

Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
Phosphorylated upon DNA damage, probably by ATM or ATR.

Cellular localization

Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.

Target information above from: UniProt accessionQ9UIS9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CXXC 3 antibody
  • CXXC-type zinc finger protein 3 antibody
  • CXXC3 antibody
  • MBD 1 antibody
  • MBD1 antibody
  • MBD1_HUMAN antibody
  • MECP1 COMPLEX antibody
  • Methyl CpG binding domain protein 1 antibody
  • Methyl CpG binding domain protein 1 antibody
  • Methyl CpG binding domain protein 1 isoform PCM1 antibody
  • Methyl CpG binding protein MBD1 antibody
  • Methyl CpG binding protein splice variant 1 antibody
  • Methyl CpG binding protein splice variant 2 antibody
  • Methyl CpG binding protein splice variant 3 antibody
  • Methyl CpG binding protein splice variant 4 antibody
  • Methyl-CpG-binding domain protein 1 antibody
  • Methyl-CpG-binding protein MBD1 antibody
  • PCM 1 antibody
  • PCM 1 antibody
  • PCM1 antibody
  • Protein containing methyl CpG binding domain 1 antibody
  • Protein containing methyl-CpG-binding domain 1 antibody
  • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
  • RFT antibody
  • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
see all

Database links

see all

Anti-MBD1 antibody - ChIP Grade images:

  Western blot - MBD1 antibody - ChIP Grade (ab3753)

Western blot - MBD1 antibody - ChIP Grade (ab3753)



Predicted band size : 57.6 kDa


1. Western blot using Rabbit polyclonal to MBD 1 at a 1/1000 dilution MCF 7 cell lysate. The antibody detects a band at approximately 60 kDa.

2. Immunolocalisation of MBD 1 in PAM212 cells.

References for Anti-MBD1 antibody - ChIP Grade (ab3753)

This product has been referenced in:

  • Nandakumar Vet al. Aberrant DNA hypermethylation patterns lead to transcriptional silencing of tumor suppressor genes in UVB-exposed skin and UVB-induced skin tumors of mice. Carcinogenesis 32:597-604 (2011).Read more (PubMed: 21186298) »
  • Chatagnon Aet al. A role for methyl-CpG binding domain protein 2 in the modulation of the estrogen response of pS2/TFF1 gene. PLoS One 5:e9665 (2010). ChIP; Human.Read more (PubMed: 20300195) »

See all 10 publications for this product

Publishing research using ab3753? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"