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Overview
Product name
Anti-MBD1 antibody
See all MBD1 products (8) ...
Description
Rabbit polyclonal to MBD1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptides RQVGPQSGEVKEAPR and PEEDKEENKDDSASK, corresponding to amino acids 98-113 and 342-356 of Human MBD1, respectively.
RQVGPQSGEV KEAPR
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS,
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
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Western blot - Anti-MBD1 antibody (ab13648)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).
Target
Function
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
Tissue specificity
Widely expressed.
Sequence similarities
Contains 3 CXXC-type zinc fingers.
Contains 1 MBD (methyl-CpG-binding) domain.
Domain
The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
Post-translational
modifications
Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
Target information above from: UniProt accessionQ9UIS9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CXXC 3 antibody
- CXXC-type zinc finger protein 3 antibody
- CXXC3 antibody
see all
Database links
- Entrez Gene: 4152 Human
- Omim: 156535 Human
- SwissProt: Q9UIS9 Human
- Unigene: 405610 Human
Anti-MBD1 antibody images:
Western blot - Anti-MBD1 antibody (ab13648)
Predicted band size : 60 kDa
Western blot analysis of MBD1 in HeLa cell lysate with anti-MBD1 antibody. A protein band with an approximate molecular weight of 55 kDa was detected.
References for Anti-MBD1 antibody (ab13648)
ab13648 has not yet been referenced specifically in any publications.
Publishing research using ab13648? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"