Anti-MBD2 Interacting Zinc Finger MIZF antibody (ab87095)

Overview

• Product nameAnti-MBD2 Interacting Zinc Finger MIZF antibody
• Description
  Rabbit polyclonal to MBD2 Interacting Zinc Finger MIZF
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
• Immunogen

  Synthetic peptide from within residues: FKDRSKLREH LRSHTQEKVV ACPTCGGMFA NNTKFLDHIR RQTSLDQQHF, corresponding to internal sequence amino acids 180-229 of Human MBD2 Interacting Zinc Finger MIZF (NP_945322)

  Run BLAST with Run BLAST with
• Positive controlTransfected 293T cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA methylation
  • Methylated DNA Binding

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

Applications

Target

• FunctionTranscriptional repressor that binds to the consensus sequence 5'-CGGACGTT-3' and to the RB1 promoter. Transcriptional activator that promotes histone H4 gene transcription at the G1/S phase transition in conjunction with NPAT. Also activates transcription of the ATM and PRKDC genes. Autoregulates its expression by associating with its own promoter.
• Tissue specificityUbiquitous. Highly expressed in brain, heart, skeletal muscle, spleen, kidney, small intestine, placenta and liver.
• Sequence similaritiesContains 9 C2H2-type zinc fingers.
• Post-translational
  modificationsUbiquitinated. Ubiquitination may lead to proteasome-mediated degradation.
• Cellular localizationNucleus. Associated with discrete nuclear foci.

Target information above from: UniProt accession Q9BQA5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 511965 Cow
  • Entrez Gene: 25988 Human
  • Entrez Gene: 25988 Human
  • Entrez Gene: 25988 Human
  • Entrez Gene: 102423 Mouse
  • Entrez Gene: 300665 Rat
  • Entrez Gene: 406733 Zebrafish
  • Omim: 607099 Human

  • SwissProt: Q2TBP2 Cow
  • SwissProt: Q9BQA5 Human
  • SwissProt: Q9BQA5 Human
  • SwissProt: Q9BQA5 Human
  • SwissProt: Q4FK13 Mouse
  • SwissProt: Q8K1K9 Mouse
  • SwissProt: Q6PFN8 Zebrafish
  • SwissProt: Q6DRP5 Zebrafish
  • Unigene: 504091 Human
  • Unigene: 24570 Mouse

  see all

• Alternative names
  DKFZp434F162 antibodyHiNF-P antibodyHINFP antibody

  HINFP_HUMAN antibodyHistone H4 transcription factor antibodyHistone nuclear factor P antibodyMBD2 interacting zinc finger protein antibodyMBD2-interacting zinc finger protein antibodyMethyl CpG binding protein 2 interacting zinc finger protein antibodyMethyl-CpG-binding protein 2-interacting zinc finger protein antibodyMIZF antibodyZNF743 antibody

  see all

Anti-MBD2 Interacting Zinc Finger MIZF antibody images

• 

  Western blot - MBD2 Interacting Zinc Finger MIZF antibody (ab87095)
  Anti-MBD2 Interacting Zinc Finger MIZF antibody (ab87095) at 1 µg/ml + Transfected 293T cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 60 kDa
  Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.