Overview
- Product nameAnti-MBD2 + MBD3 antibodySee all MBD2 + MBD3 primary antibodies ...
- DescriptionRabbit polyclonal to MBD2 + MBD3
- SpecificityThis antibody recognises the two Drosophila MBD 2 + 3 variants.
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Fruit fly (Drosophila melanogaster)
- Immunogen
Recombinant full length protein (Drosophilia melanogaster).
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferserum with 0.02% sodium azide
- PurityWhole antiserum
- Primary antibody notes Methyl CpG binding domain proteins (MBDs) are a family of mammalian proteins that specifically bind methylated regions of DNA. There are two methyl CpG binding complexes, MeCP1 and MeCP2. MeCP2 is comprised of a single protein also denoted MeCP2. MeCP1 is composed of four different proteins termed MBD 1 (also known as PCM 1), MBD 2, MBD 3, and MBD 4 (also known as MED 1).
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab3757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | |
| WB |
WB: 1/500. Detects two bands of approximately 25 kDa and 35 kDa which correspond to the predicted molecular weights of recombinant drosophila MBD 2 + 3 short and long forms, respectively.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- RelevanceDNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
- Cellular localizationNuclear
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Alternative names
- Demethylase antibodyDMTase antibodyMBD 2 antibody
- MBD 3 antibodyMethyl CpG binding domain protein 2 antibodyMethyl CpG binding domain protein 3 antibodyMethyl CpG binding protein MBD2 antibodyMethyl CpG binding protein MBD3 antibody
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Anti-MBD2 + MBD3 antibody images
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Western blot - MBD2 + MBD3 antibody (ab3757)
Predicted band size : 25-35 kDa
Rabbit polyclonal to Drosophila MBD 2 + 3 (at a 1/500 dilution):
1. Recombinant drosophila MBD 2 + 3 (long form)
2. Recombinant drosophila MBD 2 + 3d (short form)
3. Nuclear extract of Drosophila embryos where the two drosophila MBD 2 + 3 forms are detected.
References for Anti-MBD2 + MBD3 antibody (ab3757)
ab3757 has not yet been referenced specifically in any publications.
