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Western blot - MBD2 antibody (ab38646)
(enlarge)
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Product Name
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MBD2 antibody
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See all MBD2 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MBD2
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Immunogen
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A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 160-177 of MBD2 (Human)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody is specific for MBD2 (N term).
Rat - Abreview feedback has stated that this antibody works on rat prostate. It gives some non-specific band in rat testis, probably due to presence of other MBD isoforms.
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
ICC/IF: 1/50.
WB: 1/100 - 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A375 cell lysate and mouse brain tissue lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methylated DNA
Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA
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Relevance
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DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. This protein may function as a mediator of the biological consequences of the methylation signal.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody was eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MBD2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MBD2 antibody images, references, products related to ab38646 and other tools.
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MBD2 antibody images:
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 Western blot - MBD2 antibody (ab38646)
All lanes : MBD2 antibody (ab38646)
Lane 1 : A375 cell lysate
Lane 2 : mouse brain tissue lysate
Predicted band size : 43 kDa
Observed band size : 43 kDa
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
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References for MBD2 antibody (ab38646)
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This product has been used in:
Burd CJ et al. UV radiation regulates Mi-2 through protein translation and stability. J Biol Chem 283:34976-82 (2008). WB; Human. PubMed: 18922793
If you publish research using ab38646 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MBD2
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MBD2 antibody - more information
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Customer reviews (feedback) regarding MBD2 antibody |
Customer FAQs regarding MBD2 antibody |
Protocols for MBD2 antibody |
Price and availability of products related to MBD2 antibody |
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MBD2 antibody - related products:
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MBD2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MBD2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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