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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA
Anti-MBD2 antibody
See all MBD2 products (9) ...
Rabbit polyclonal to MBD2
This antibody is specific for MBD2 (N term). Rat - Abreview feedback has stated that this antibody works on rat prostate. It gives some non-specific band in rat testis, probably due to presence of other MBD isoforms.
ICC/IF, ELISA, WBmore details
Reacts with
Mouse, Rat, Human
A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 160-177 of MBD2 (Human)
A375 cell lysate and mouse brain tissue lysate.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration information loading...
Protein G purified
This antibody was eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Polyclonal
IgG
Our Abpromise guarantee covers the use of ab38646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/50
ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemi-methylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Isoform 1 may enhance the activation of some unmethylated cAMP-responsive promoters. Reports about DNA demethylase activity of isoform 2 are contradictory.
Highly expressed in brain, heart, kidney, stomach, testis and placenta.
Contains 1 MBD (methyl-CpG-binding) domain.
Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
Target information above from: UniProt accessionQ9UBB5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - MBD2 antibody (ab38646)

All lanes : Anti-MBD2 antibody (ab38646)
Lane 1 : A375 cell lysate
Lane 2 : mouse brain tissue lysate
Predicted band size : 43 kDa
Observed band size : 43 kDa
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - MBD2 antibody (ab38646)

ab38646 staining MBD2 in rat liver cultured cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 10 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/400 dilution.
Image courtesy of an anonymous Abreview.
This product has been referenced in:
See 1 publication for this product
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All lanes : Anti-MBD2 antibody (ab38646)
Lane 1 : A375 cell lysate
Lane 2 : mouse brain tissue lysate
Predicted band size : 43 kDa
Observed band size : 43 kDa
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

ab38646 staining MBD2 in rat liver cultured cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 10 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/400 dilution.
Image courtesy of an anonymous Abreview.
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