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Products:Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA
Overview
Product name
Anti-MBD2 antibody
See all MBD2 products (9) ...
Description
Goat polyclonal to MBD2
Specificity
Expected to recognize isoform 1 only.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide: RNDPLNQNKGKPDLN, corresponding to internal sequence amino acids 226-240 of Human MBD2
RNDPLNQNKG KPDLN
Positive control
Jurkat cell lysate Human colon
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1.0 µg/ml.Predicted molecular weight: 43 kDa.
IHC-P: Use a concentration of 2 - 4 µg/ml.
Target
Function
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemi-methylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Isoform 1 may enhance the activation of some unmethylated cAMP-responsive promoters. Reports about DNA demethylase activity of isoform 2 are contradictory.
Tissue specificity
Highly expressed in brain, heart, kidney, stomach, testis and placenta.
Sequence similarities
Contains 1 MBD (methyl-CpG-binding) domain.
Cellular localization
Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
Target information above from: UniProt accessionQ9UBB5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DEMETHYLASE antibody
- DKFZp586O0821 antibody
- DMTase antibody
see all
Database links
- Entrez Gene: 8932 Human
- Entrez Gene: 17191 Mouse
- Omim: 603547 Human
- SwissProt: Q9UBB5 Human
- SwissProt: Q9Z2E1 Mouse
- SwissProt: Q8AYT1 Xenopus laevis
- SwissProt: Q7ZTV3 Zebrafish
- Unigene: 25674 Human
see all
Anti-MBD2 antibody images:
Western blot - MBD2 antibody (ab58241)
Anti-MBD2 antibody (ab58241) at 0.3 µg/ml + Jurkat cell lysate at 35 µg
Predicted band size : 43 kDa
Observed band size : 43 kDa
Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody (ab58241)
ab58241 at 3.5 µg/ml staining MBD2 in Human colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody revealed by AP-staining.
References for Anti-MBD2 antibody (ab58241)
This product has been referenced in:
- Müller Iet al. Promoter- and cell-specific epigenetic regulation of CD44, Cyclin D2, GLIPR1 and PTEN by methyl-CpG binding proteins and histone modifications. BMC Cancer 10:297 (2010). WB; Mouse.Read more (PubMed: 20565761) »
See 1 publication for this product
Publishing research using ab58241? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"