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All lanes : Anti-MBD3 antibody - Carboxyterminal end (ab71777) at 1/100 dilution
Lane 1 : Non-transfected 293 cell lysates
Lane 2 : MBD3 transfected 293 cell lysates
Lysates/proteins at 2 µg per lane.
Predicted band size : 33 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71777 at a 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.
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